Scopus
YÖKSİS Eşleşti
From parasitic life to health-promoting applications - A versatile goldmine discovered in nature's secret treasure chest: Orobanche nana
Food Bioscience · Aralık 2024
YÖKSİS Kayıtları
From parasitic life to health-promoting applications - a versatile goldmine discovered in natures secret treasure chest: Orobanche nana
Food Bioscience · 2024 SCI-Expanded
PROFESÖR EVREN YILDIZTUGAY →
Makale Bilgileri
DergiFood Bioscience
Yayın TarihiAralık 2024
Cilt / Sayfa62
Scopus ID2-s2.0-85206801629
Özet
Studies evaluating the phytoconstituents and therapeutic potential of species belonging to Orobanche (Family Orobanchaceae) are rather limited. The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of the aerial parts of Orobanche nana Noë ex Rchb. Extracts were prepared by sequential maceration in dichloromethane, ethyl acetate and ethanol and finally an aqueous extraction by infusion were performed. NMR and LC-DAD-MS analysis allowed the identification of different phytoconstituents including the characteristic compounds of Orobanche like verbascoside, poliumoside, orobanone and orobanchoside. The ethanol extract displayed the highest total phenolic (115.63 mg GAE/g) and flavonoids (16.53 mg RE/g) contents, antiradical (DPPH = 623.70 mg TE/g; ATBS = 843.50 mg TE/g) and ions reducing (Cu++ = 725.39; Fe+++ = 617.70) properties while the aqueous extract exerted the best chelating power (19.54 mg EDTAE/g). It was determined that EtOH and Water extracts applied to HepG2 cells decreased the levels of anti-apoptotic proteins p-NFκB, BCL-2 and BCL-XL, while increasing the levels of apoptotic proteins BAX and p-53, thus inhibiting cell survival. In addition, Orobanche nana played an active role in the rearrangement of overexpress MMPs that cause disruption of ECM homeostasis. We employed Network Pharmacology to investigate the mechanisms through which Orobanche nana's compounds impact Kidney and Liver diseases. These findings suggested that O. nana could be a promising source of bioactive molecules with potential therapeutic applications.
Yazarlar (11)
1
Inci Kurt-Celep
2
Sakina Yagi
3
Stefano Dall'Acqua
4
Stefania Sut
5
Engin Celep
6
Savas Kaya
7
Avni Berisha
8
Sathish Kumar M. Ponnaiya
9
Kamalavarshini Balakrishnan
10
Evren Yildiztugay
ORCID: 0000-0002-4675-2027
11
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
antioxidant
Cytotoxicity
Enzyme inhibition
Orobanche nana
Phytoconstituents
Kurumlar
Acıbadem Mehmet Ali Aydınlar Üniversitesi
Istanbul Turkey
Cumhuriyet Üniversitesi
Sivas Turkey
Khartoum University
Khartoum Sudan
Okan Üniversitesi
Tuzla Turkey
Ponnaiya's Code and Genome Pvt Ltd
Madurai India
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Padova
Padua Italy
Université de Lorraine
Nancy France
Universiteti i Prishtinës
Pristina Serbia