Scopus
YÖKSİS Eşleşti
Integration Viewpoint Using UHPLC-MS/MS, In Silico Analysis, Network Pharmacology, and In Vitro Analysis to Evaluate the Bio-Potential of Muscari armeniacum Extracts
Molecules · Temmuz 2025
YÖKSİS Kayıtları
Integration viewpoint using UHPLC-MS/MS, in silico, network pharmacology and in vitro analysis to evaluate the bio-potential of Muscari
armeniacum extracts
Molecules · 2025 SCI-Expanded
PROFESÖR EVREN YILDIZTUGAY →
Makale Bilgileri
DergiMolecules
Yayın TarihiTemmuz 2025
Cilt / Sayfa30
Scopus ID2-s2.0-105010333689
Özet
The current study investigates the chemical profiling, antioxidant activities, and enzyme inhibitory and cytotoxic potential of the water and methanolic extracts of different parts (flower, leaf, and bulb) of Muscari armeniacum. Chemical profiling was performed using UHPLC-MS/MS. At the same time, different in vitro assays were employed to support the results for antioxidant potential, such as DPPH, ABTS, FRAP, CUPRAC, metal chelation, and PBD, along with the measurement of total phenolic and flavonoid contents. Enzyme inhibition was investigated for cholinesterase (AChE and BChE), α-amylase, α-glucosidase, and tyrosinase enzymes. Additionally, the relative expression of NRF2, HMOX1, and YGS was evaluated by qPCR. LC-MS/MS analysis indicated the presence of some significant compounds, including apigenin, muscaroside, hyacinthacine A, B, and C, and luteolin. According to the results, the highest TPC and TFC were obtained with both extracts of the leaves, followed by the water extract (flower) and methanolic extract of the bulb. In contrast, the methanolic extract from the bulb exhibited the highest antioxidant potential using DPPH, ABTS, CUPRAC, and FRAP, followed by the extracts of leaves. In contrast, the leaf extracts had the highest values for the PBD assay and maximum chelation ability compared to other tested extracts. According to the enzyme inhibition studies, the methanolic extract from the bulb appeared to be the most potent inhibitor for all the tested enzymes, with the highest values obtained for AChE (1.96 ± 0.05), BChE (2.19 ± 0.33), α-amylase (0.56 ± 0.02), α-glucosidase (2.32 ± 0.01), and tyrosinase (57.19 ± 0.87). Interestingly, the water extract from the bulb did not inhibit most of the tested enzymes. The relative expression of NRF2 based on qPCR analysis was considerably greater in the flower methanol extract compared to the other extracts (p < 0.05). The relative expression of HMOX1 was stable in all the extracts, whereas YGS expression remained stable in all the treatments and had no statistical differences. The current results indicate that the components of M. armeniacum (leaves, flowers, and bulb) may be a useful source of natural bioactive compounds that are effective against oxidative stress-related conditions, including hyperglycemia, skin disorders, and neurodegenerative diseases. Complementary in silico approaches, including molecular docking, dynamics simulations, and transcription factor (TF) network analysis for NFE2L2, supported the experimental findings and suggested possible multi-target interactions for the selected compounds.
Yazarlar (10)
1
Nilufar Nilofar
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Mehmet Veysi Cetiz
ORCID: 0000-0002-2635-6553
4
Evren Yildiztugay
ORCID: 0000-0002-4675-2027
5
Zoltán Cziáky
6
József Jekő
7
Claudio Ferrante
8
Tina Kostka
9
Tuba Esatbeyoglu
10
Stefano Dall’Acqua
ORCID: 0000-0001-8264-6953
Anahtar Kelimeler
antioxidant
bioactive agents
enzyme inhibition
in sillico
Muscari armeniacum
muscaroside
NRF2
Kurumlar
Gottfried Wilhelm Leibniz Universität Hannover
Hannover Germany
Harran University, Faculty of Medicine
Sanliurfa Turkey
Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau
Kaiserslautern Germany
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Padova
Padua Italy
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Nyíregyháza
Nyíregyháza Hungary