Scopus
YÖKSİS Eşleşti
The Effects of Engeletin on Insulin Resistance Induced in Human HepG2 Liver Cells
Current Issues in Molecular Biology · Temmuz 2025
YÖKSİS Kayıtları
The Effects of Engeletin on Insulin Resistance Induced in Human HepG2 Liver Cells
Current Issues in Molecular Biology · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUHAMMED YAYLA →
Makale Bilgileri
DergiCurrent Issues in Molecular Biology
Yayın TarihiTemmuz 2025
Cilt / Sayfa47
Scopus ID2-s2.0-105011739719
Özet
In this study, we aimed to investigate the effect of Engeletin (ENG) on insulin resistance and the associated oxidative cell damage in human HepG2 liver cells. The cells were grown in a cell culture medium, and insulin resistance was induced. After the determination of the toxic and effective doses of Engeletin, the effects of Engeletin on insulin resistance and insulin resistance-induced oxidative damage, inflammation, and apoptosis. To induce IR, culture plates were treated with 30 mM glucose and 50 nM insulin and incubated for 48 h. Engeletin and metformin were given one hour before starting the insulin resistance induction. In the HepG2 cells, insulin resistance decreased glucose consumption, the expression of ISR-1 and ISR-2, and the GLUT-2 levels, while they were all increased by Engeletin, which showed a metformin-like effect. In addition, Engeletin alleviated oxidative cell damage by decreasing MDA levels, which increased due to insulin resistance-induced oxidative stress, increasing the GSH and SOD levels and decreasing the caspase-3 (Cas-3), caspase-9 (Cas-9), and tumor necrosis factor alpha (TNF-α) levels, which also increase under insulin resistance conditions. Engeletin was found to have the protective and therapeutic effect of reducing insulin resistance (IR) and the oxidative cell damage it causes in human HepG2 cells.
Yazarlar (6)
1
Erdem Toktay
ORCID: 0000-0002-0715-2707
2
Secil N. Parlak
3
Tugba Kavas
4
Harun Un
ORCID: 0000-0003-1772-282X
5
Rustem Anil Ugan
ORCID: 0000-0002-4837-2343
6
Muhammed Yayla
ORCID: 0000-0002-0659-3084
Anahtar Kelimeler
Engeletin
flavonoids
GLUT-2
insulin resistance
ISR-1
ISR-2
oxidative stress
Kurumlar
Aǧrı İbrahim Çeçen Üniversitesi
Agri Turkey
Atatürk Üniversitesi
Erzurum Turkey
Kafkas Üniversitesi
Kars Turkey
Selçuk Tip Fakültesi
Konya Turkey