Scopus
YÖKSİS Eşleşti
Different duration of exposure to a pulsed magnetic field can cause changes in mRNA expression of apoptotic genes in oleic acid-treated neuroblastoma cells
International Journal of Radiation Biology · Ocak 2024
YÖKSİS Kayıtları
Different duration of exposure to a pulsed magnetic field can cause changes in mRNA expression of apoptotic genes in oleic acid-treated neuroblastoma cells
International Journal of Radiation Biology · 2024 SCI-Expanded
PROFESÖR SERDAR KARAKURT →
Makale Bilgileri
DergiInternational Journal of Radiation Biology
Yayın TarihiOcak 2024
Cilt / Sayfa100 · 1471-1480
Scopus ID2-s2.0-85200235391
Özet
Purpose: Neuroblastoma, a prevalent childhood tumor, poses significant challenges in therapeutic interventions, especially for high-risk cases. This study aims to fill a crucial gap in our understanding of neuroblastoma treatment by investigating the potential molecular impacts of short- and long-term pulsed magnetic field exposure on the neuronal apoptosis mechanism in an in vitro model of neuroblastoma treated with oleic acid (OA). Materials and methods: Cells were cultured and divided into six following experimental groups: (I) Nontreated group (NT); (II) OA-treated group (OA); (III) Group treated with OA after being exposed to the pulsed magnetic field for 15-min (15 min PEMF + OA); (IV) Group treated with OA after being exposed to the pulsed magnetic field for 12 h (12 h PEMF + OA); (V) Group exposed to the pulsed magnetic field for 15 min (15 min PEMF); and (VI) Group exposed to the pulsed magnetic field for 12 h (12 h PEMF). Cell viability, rates of apoptosis, and mRNA levels of key apoptotic genes (TP53, Bcl2, Bax, and Caspase-3) were assessed. Results: Significant reductions in cell viability were observed, particularly in the group treated with OA following long-term pulsed magnetic field exposure. Flow cytometry revealed elevated apoptosis rates, notably in the early stages of apoptosis. qRT-PCR analysis demonstrated increased expression of cleaved Caspase-3, Bax/Bcl2 ratio, and TP53 in cells treated with OA following long-term pulsed magnetic field exposure, signifying enhanced apoptotic pathways. Conclusions: The findings indicate that long-term pulsed magnetic field exposure and OA treatment exhibit potential synergistic effects leading to the induction of apoptosis in SH-SY5Y cells. We have concluded that stimulations of pulsed magnetic field have the potential to serve as an adjuvant therapy for oleic acid-based treatment of neuroblastoma.
Yazarlar (5)
1
Çiğdem Gökçek-Saraç
ORCID: 0000-0002-3538-6551
2
Ebru Çetin
ORCID: 0000-0001-8675-5479
3
Kayhan Ateş
ORCID: 0000-0002-6016-6577
4
Şükrü Özen
ORCID: 0000-0002-5538-6786
5
Serdar Karakurt
Anahtar Kelimeler
apoptosis
neuroblastoma
oleic acid
Pulsed magnetic field
Kurumlar
Akdeniz Üniversitesi
Antalya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Pavia
Pavia Italy