Scopus
YÖKSİS DOI Eşleşti
SJR Q2
Different duration of exposure to a pulsed magnetic field can cause changes in mRNA expression of apoptotic genes in oleic acid-treated neuroblastoma cells
International Journal of Radiation Biology · Ocak 2024
YÖKSİS Kayıtları
Different duration of exposure to a pulsed magnetic field can cause changes in mRNA expression of apoptotic genes in oleic acid-treated neuroblastoma cells
International Journal of Radiation Biology · 2024 SCI-Expanded
Prof. Dr. SERDAR KARAKURT →
YÖKSİS Kayıtları — ISSN Eşleşmesi
investigation of the protective effect of melatonin againts radiobiological damage in laryngeal tissue due to radiotherapy at different dose rates in rats
2026 ISSN: 0955-3002 SCI-Expanded
Prof. Dr. FATMA HÜMEYRA YERLİKAYA AYDEMİR →
Different duration of exposure to a pulsed magnetic field can cause changes in mRNA expression of apoptotic genes in oleic acid-treated neuroblastoma cells
2024 ISSN: 0955-3002 SCI-Expanded Q1
Prof. Dr. SERDAR KARAKURT →
Makale Bilgileri
ISSN09553002
Yayın TarihiOcak 2024
Cilt / Sayfa100 · 1471-1480
Scopus ID2-s2.0-85200235391
Özet
Purpose: Neuroblastoma, a prevalent childhood tumor, poses significant challenges in therapeutic interventions, especially for high-risk cases. This study aims to fill a crucial gap in our understanding of neuroblastoma treatment by investigating the potential molecular impacts of short- and long-term pulsed magnetic field exposure on the neuronal apoptosis mechanism in an in vitro model of neuroblastoma treated with oleic acid (OA). Materials and methods: Cells were cultured and divided into six following experimental groups: (I) Nontreated group (NT); (II) OA-treated group (OA); (III) Group treated with OA after being exposed to the pulsed magnetic field for 15-min (15 min PEMF + OA); (IV) Group treated with OA after being exposed to the pulsed magnetic field for 12 h (12 h PEMF + OA); (V) Group exposed to the pulsed magnetic field for 15 min (15 min PEMF); and (VI) Group exposed to the pulsed magnetic field for 12 h (12 h PEMF). Cell viability, rates of apoptosis, and mRNA levels of key apoptotic genes (TP53, Bcl2, Bax, and Caspase-3) were assessed. Results: Significant reductions in cell viability were observed, particularly in the group treated with OA following long-term pulsed magnetic field exposure. Flow cytometry revealed elevated apoptosis rates, notably in the early stages of apoptosis. qRT-PCR analysis demonstrated increased expression of cleaved Caspase-3, Bax/Bcl2 ratio, and TP53 in cells treated with OA following long-term pulsed magnetic field exposure, signifying enhanced apoptotic pathways. Conclusions: The findings indicate that long-term pulsed magnetic field exposure and OA treatment exhibit potential synergistic effects leading to the induction of apoptosis in SH-SY5Y cells. We have concluded that stimulations of pulsed magnetic field have the potential to serve as an adjuvant therapy for oleic acid-based treatment of neuroblastoma.
Yazarlar (5)
1
Çiğdem Gökçek-Saraç
ORCID: 0000-0002-3538-6551
2
Ebru Çetin
ORCID: 0000-0001-8675-5479
3
Kayhan Ateş
ORCID: 0000-0002-6016-6577
4
Şükrü Özen
ORCID: 0000-0002-5538-6786
5
Serdar Karakurt
Anahtar Kelimeler
apoptosis
neuroblastoma
oleic acid
Pulsed magnetic field
Kurumlar
Akdeniz Üniversitesi
Antalya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Pavia
Pavia Italy
Scimago Dergi (ISSN Eşleşmesi)
International Journal of Radiation Biology
Q2
SJR Skoru0,553
H-Index104
YayıncıInforma Healthcare
ÜlkeUnited Kingdom
Radiological and Ultrasound Technology (Q2)
Radiology, Nuclear Medicine and Imaging (Q2)
Metrikler
1
Atıf
5
Yazar
4
Anahtar Kelime