Scopus
YÖKSİS Eşleşti
Increased Fat Graft Survival with Mesenchymal Stem Cell Recruiting Effect of PRP: In Vitro and In Vivo Study of Application Techniques
Genel Tip Dergisi · Nisan 2022
YÖKSİS Kayıtları
Increased fat graft survival with mesenchymal stem cell recruiting effect of PRP: In vitro and in vivo study of application techniques
Selcuk Universitesi Genel Tıp Dergisi · 2022 TR DİZİN
PROFESÖR HASİBE ARTAÇ →
Increased fat graft survival with mesenchymal stem cell recruiting effect of PRP: in vitro and in vivo study of application techniques
Genel Tıp Dergisi · 2022 EBSCOHost, GoogleScholar, OpenAIRE
DOÇENT GÖKÇE YILDIRAN →
Increased fat graft survival with mesenchymal stem cell recruiting effect of PRP: in vitro and in vivo study of application techniques
Genel Tıp Dergisi · 2022 TR DİZİN
DOÇENT HÜLYA ÖZDEMİR →
Makale Bilgileri
DergiGenel Tip Dergisi
Yayın TarihiNisan 2022
Cilt / Sayfa32 · 207-214
Scopus ID2-s2.0-105009422050
Özet
Aim: Adding platelet-rich plasm (PRP) or mesenchymal stem cells are the most accepted methods to increase fat graft’s permanence. However, there is no consensus on timing and whether the effect of stem cells or PRP is observed more in the recipient area or in the donor area. It is aimed to present the application method and localization of PRP to be combined with fat graft to increase the survival. Material and Methods: in vitro part: Fat grafts were kept in a medium containing PRP. Cell output from the fat graft to the Petri dishes was examined every day. The time to recruit the maximum number of stem cells to the medium was determined (day 10). in vivo part: Eight group of rats were received PRP in different concentrations either to recipient or donor areas of fat grafts. After ten days, fat grafts were transferred to the recipient area. Ninety days after the transfer, histopathological examination and immunohistochemical staining were performed. Results: The group which had received the full-dose PRP to the recipient area showed the highest cellular integrity and vascularity (p< 0.05). Vascularity was superior in the half-dose PRP group compared to the control group (p< 0.05); cellular integrity did not increase. Donor site groups did not show increase in cellular integrity. Conclusion: Although PRP starts to increase fat graft permanence by increasing vascularity, it acts by preserving the cellular integrity of the fat cells as the concentrations increase. The PRP injection to the recipient area 10 days before fat grafting may provide higher survival rates.
Yazarlar (10)
1
Gokce Yildiran
2
Osman Akdag
3
Gulsemin Cicek
ORCID: 0000-0003-2399-6755
4
Zeliha Esin Celik
5
Hasibe Artac
6
Hülya Uçaryilmaz
7
Selcuk Duman
8
Mustafa Sutcu
9
Tahsin Murad Aktan
10
Zekeriya Tosun
Anahtar Kelimeler
fat graft
PRP
stem cell
survival
Kurumlar
Kanuni Sultan Suleyman Training and Research Hospital
Istanbul Turkey
Konya City Hospital
Konya Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
2
Atıf
10
Yazar
4
Anahtar Kelime