Scopus
YÖKSİS Eşleşti
A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot-modified carbon paste electrode
Biotechnology and Applied Biochemistry · Şubat 2021
YÖKSİS Kayıtları
A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot-modified carbon paste electrode
Biotechnology and Applied Biochemistry · 2021 SCI-Expanded
DOÇENT SALİHA DİNÇ →
A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot-modified carbon paste electrode
Biotechnology and Applied Biochemistry · 2021 SCI-Expanded
PROFESÖR MUSTAFA ÖZMEN →
Makale Bilgileri
DergiBiotechnology and Applied Biochemistry
Yayın TarihiŞubat 2021
Cilt / Sayfa68 · 20-29
Scopus ID2-s2.0-85079707986
Özet
Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer‘s disease. That is why it is an important agent for this disease. In this study, a bienzymatic biosensor system with acetylcholine esterase and choline oxidase was prepared with carbon paste electrode modified with carbon nano Dot-(3-Aminopropyl) triethoxysilane (CDs-APTES) for determination of the amount of acetylcholine. Acetylcholine esterase and choline oxidase enzymes were immobilized onto a modified carbon paste electrode by cross-linking with glutaraldehyde. Determination of acetylcholine was carried out by the oxidation of enzymatically produced H<inf>2</inf>O<inf>2</inf> at 0.4 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the acetylcholine response of the prepared biosensor was investigated. In addition, the optimum CDs-APTES amount, the linear operating range of the biosensor, and the interference effect were also investigated.
Yazarlar (4)
1
Onur Can Bodur
2
Saliha Dinç
3
Mustafa Ozmen
ORCID: 0000-0001-5117-9168
4
Fatma Arslan
Anahtar Kelimeler
acetylcholine esterase
biosensor
choline oxidase
Kurumlar
Gazi Üniversitesi
Ankara Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
29
Atıf
4
Yazar
3
Anahtar Kelime