Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Highly sensitive detection of Campylobacter jejuni using a carbon dot-embedded nanoMIPs fluorescent sensor
Microchemical Journal · Ocak 2025
YÖKSİS Kayıtları
Highly sensitive detection of Campylobacter jejuni using a carbon dot-embedded nanoMIPs fluorescent sensor
Microchemical Journal · 2025 SCI-Expanded
DOÇENT SALİHA DİNÇ →
Makale Bilgileri
DergiMicrochemical Journal
Yayın TarihiOcak 2025
Cilt / Sayfa208
Scopus ID2-s2.0-85210356502
Erişim🔓 Açık Erişim
Özet
Campylobacter jejuni, a leading cause of gastroenteritis worldwide, poses significant detection challenges due to the labor-intensive and insufficiently sensitive current methods. Developing efficient, rapid diagnostics is vital for clinical and food industry applications; yet the vulnerability of biological elements in creating bacterial sensors remains a major obstacle. This study introduces an innovative fluorescence-based sensor employing fully synthetic carbon dot (CDs) functionalized molecularly imprinted polymer (CDs@nanoMIPs) receptors to detect this foodborne pathogenic bacterium. The CDs@nanoMIPs synthesis was accomplished using a solid-phase approach, with an immunodominant epitope of C. jejuni serving as a guiding template. During the polymerization process, nitrogen-doped CDs were synthesized in situ and incorporated into nanoMIPs as a fluorescent tag, constituting approximately 19 % of the composite. The synthesized nanomaterials were characterized by employing transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR), fluorescence microscopy, UV–Vis spectrophotometry, and fluorescence spectrometry. CDs@nanoMIPs demonstrated a notable blue fluorescence when excited at 350 nm, along with excellent photostability and a negative zeta potential. These nanostructured particles (59 nm) exhibited moderate polydispersity and a spherical morphology. The FT-IR spectrum deviated from that of conventional CDs, aligning more closely with the template epitope. When in contact with C. jejuni, CDs@nanoMIPs induced a significant increase in fluorescence intensity, enabling the efficient bacterial detection. This interaction showed exceptional affinity and sensitivity towards C. jejuni, featuring a linear range of 1 × 10<sup>1</sup>–1 × 10<sup>8</sup> CFU mL<sup>−1</sup> (R<sup>2</sup> = 0.98) and a low detection limit of 4.6 CFU mL<sup>−1</sup>. CDs@nanoMIPs-based C. jejuni sensors marked a novel approach to pathogen detection.
Yazarlar (6)
1
Saliha Dinç
2
Tim Tjardts
3
Gregor Maschkowitz
4
Vivian Lukaszczuk
5
Seyed Mohammad Taghi Gharibzahedi
6
Zeynep Altintas
Anahtar Kelimeler
Campylobacter jejuni
Carbon dots (CDs)
CD-embedded nanoMIPs
Flagellar capping protein
Fluorescent sensor
Kurumlar
Christian-Albrechts-Universität zu Kiel
Kiel Germany
Metrikler
1
Atıf
6
Yazar
5
Anahtar Kelime