Scopus
YÖKSİS Eşleşti
From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from Helichrysum plicatum DC. subsp. plicatum
Pharmaceuticals · Haziran 2025
YÖKSİS Kayıtları
From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from Helichrysum plicatum DC. subsp. plicatum
Pharmaceuticals · 2025 SCI-Expanded
ARAŞTIRMA GÖREVLİSİ TUĞSEN BÜYÜKYILDIRIM →
Makale Bilgileri
DergiPharmaceuticals
Yayın TarihiHaziran 2025
Cilt / Sayfa18
Scopus ID2-s2.0-105009085756
Özet
Background: Varicose veins and chronic venous insufficiency are chronic venous disorders involving abnormalities in the venous system. Inflammation, an increase in proteolytic enzymes, and free radicals are important factors that play a role in the varicose vein pathology. Methods: In this study, the antioxidant properties and inhibitor activities of 17 plant extracts used to treat varicose veins in traditional medicine were evaluated against varicose veins-related enzymes (hyaluronidase, elastase, collagenase, lipooxygenase, prolylendopeptidase, and xanthine oxidase). The most effective compounds responsible for the activity of the Helichrysum plicatum subsp. plicatum extract were isolated by open column chromatography techniques. The active compounds were determined to be naringenin, apigenin, and luteolin by spectroscopic methods. In the activity-guided isolation study, the xanthine oxidase enzyme inhibition method was used. Results: The fractions containing naringenin and apigenin (IC<inf>50</inf> = 0.269 ± 0.009 µg/mL) and apigenin and luteolin (IC<inf>50</inf> = 0.285 ± 0.019 µg/mL) compounds showed synergistic and strong effects against xanthine oxidase and were found to be as active as the positive control allopurinol (IC<inf>50</inf> = 0.250 ± 0.006 µg/mL). In the LC-MS/MS analysis of the Helichrysum plicatum extract, quinic acid (22.649 mg compound/g extract), chlorogenic acid (14.573 mg/g extract), isoquercitrin (14.371 mg/g extract), cosmosin (9.885 mg/g extract), and astragalin (11.506 mg/g extract) were detected as the major components. Naringenin, apigenin, and luteolin were detected at concentrations of 1.457, 2.518, and 1.368 mg/g in the extract, respectively. Conclusions: In conclusion, it is predicted that the combination of naringenin, apigenin, and luteolin has a promising use as a conservative treatment option for diseases associated with varicose veins due to their synergistic effects with each other.
Yazarlar (6)
1
Tugsen Buyukyildirim
2
F. Sezer Senol Deniz
ORCID: 0000-0002-5850-9841
3
Merve Yuzbasioglu Baran
ORCID: 0000-0002-7482-0429
4
Ece Salihoglu
ORCID: 0000-0003-0681-3566
5
Mustafa Abdullah Yılmaz
6
Osman Tugay
Anahtar Kelimeler
antioxidant activity
apigenin
flavonoids
Helichrysum plicatum subsp. plicatum
in vitro enzyme inhibition activity
LC-MS/MS
luteolin
naringenin
varicose vein
Kurumlar
Dicle Üniversitesi
Diyarbakir Turkey
Gazi Üniversitesi
Ankara Turkey
Selçuk Üniversitesi
Selçuklu Turkey
University of Health Sciences
Istanbul Turkey