Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Berberis vulgaris fruit extract mitigated apoptosis in experimentally induced testicular ischemia and reperfusion injury in rats
Frontiers in Pharmacology · Ocak 2025
YÖKSİS Kayıtları
Berberis vulgaris fruit extract mitigated apoptosis in experimentally induced testicular ischemia and reperfusion injury in rats
Frontiers in Pharmacology · 2025 SCI
DOKTOR ÖĞRETİM ÜYESİ MUHAMMED YAYLA →
Makale Bilgileri
DergiFrontiers in Pharmacology
Yayın TarihiOcak 2025
Cilt / Sayfa16
Scopus ID2-s2.0-105002593298
Erişim🔓 Açık Erişim
Özet
Aim: The aim was to investigate the possible protective effect of Berberis vulgaris extract, which has anti-inflammatory and antioxidant properties, in the testes in an ischemia–reperfusion (IR) injury model by utilizing molecular, biochemical, histopathological, and immunohistochemical methods. Methods: A total of 56 male rats were divided in to 7 equal groups. Ischemia was induced by taking the testicles out of the scrotum and rotating them 720° and after 3 h reperfusion was induced. Berberis vulgaris extract was administered 3 h before ischemia. The reperfusion groups were treated by oral gavage 1 h before reperfusion. Following the 3-h reperfusion period, tissue and blood samples were collected. Results: Histopathologically, H&E staining showed disruption in the seminiferous tubule structure in the ischemia and IR groups SOD levels decreased significantly in the IR group compared to the control group, whereas Berberis vulgaris did not change SOD levels following IR at 300 and 600 mg/kg doses. At the dose of 600 mg/kg, Berberis vulgaris significantly increased SOD levels compared to the ischemia group CAT activity was significantly higher in the BV300 and BV600 groups compared to the ischemia group. CAT activity was significantly lower in the IR group compared to the ischemia group (p < 0.001). When compared to the control group, the ischemia group had a roughly 3-fold increase in caspase-3 expression. In the IR group, this ratio was dramatically increased—roughly 5-fold. However, the antiapoptotic gene Bcl-2 expression was significantly decreased in both the ischemia and IR groups compared to the control group. Conclusion: B. vulgaris plant extract may have a protective effect against testes IR injury. Further molecular studies are needed to clarify this protective effect.
Yazarlar (12)
1
Serdar Yigit
ORCID: 0000-0001-5631-2335
2
Sakir Akgun
3
Isa Yesilyurt
ORCID: 0000-0002-2164-4580
4
Soner Bitiktaş
5
Ergin Taskin
ORCID: 0000-0002-1883-6055
6
Ali Alper Kahraman
ORCID: 0000-0003-2924-2655
7
Muhammed Yayla
ORCID: 0000-0002-0659-3084
8
Pınar Aksu Kilicle
9
Seyit Ali Bingol
ORCID: 0000-0001-6422-9582
10
Bengul Ozdemir
11
Gül Esma Akdogan
12
Mehmet Ali Karagöz
Anahtar Kelimeler
bcl-2
Berberis vulgaris
caspase-3
ischemia and reperfusion
testes
Kurumlar
Aǧrı İbrahim Çeçen Üniversitesi
Agri Turkey
Faculty of Science
Kars Turkey
Kafkas Üniversitesi
Kars Turkey
Selçuk Tip Fakültesi
Konya Turkey