Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Exploration of Small Non-Coding RNAs as Molecular Markers of Ram Sperm Fertility
International Journal of Molecular Sciences · Mart 2025
YÖKSİS Kayıtları
Exploration of Small Non-Coding RNAs as Molecular Markers of Ram Sperm Fertility
International Journal of Molecular Sciences · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Makale Bilgileri
DergiInternational Journal of Molecular Sciences
Yayın TarihiMart 2025
Cilt / Sayfa26
Scopus ID2-s2.0-105002283951
Erişim🔓 Açık Erişim
Özet
The identification of molecular markers for fertility is critical for the sustainability of livestock production. We profiled small non-coding RNAs (sncRNAs) in sperm from rams with high fertility (HF) and low fertility (LF) phenotypes to uncover their roles in ram sperm fertility. Rams were categorized into high-fertility (HF, n = 31; 94.5 ± 2.8%) and low-fertility (LF, n = 25; 83.1 ± 5.73%) phenotypes based on pregnancy rates (average 89.4 ± 7.2%). From these, sperm samples of HF (n = 4; pregnancy rate 99.2 ± 1.6%) and LF (n = 4; pregnancy rate 73.6 ± 4.4%) rams underwent sncRNA sequencing. Small RNA sequencing produced 14,962,876 reads in LF rams and 17,401,094 reads in HF rams, showing distinct sncRNA biotypes, including miRNAs, tRNAs, snoRNAs, snRNAs, and rRNAs. Among these, miRNAs comprised 7.12% of reads in LF rams and 3.78% in HF rams, while rRNAs and repeats formed significant proportions in both groups. A total of 1673 known and 627 novel miRNAs were identified, with 227 differentially expressed miRNAs between the HF and LF groups. We showed that key miRNAs, such as oar-miR-200b and oar-miR-370-3p, were upregulated in HF sperm, while downregulated miRNAs in LF, such as oar-miR-26b and oar-let-7d, were associated with impaired sperm function and DNA fragmentation. A functional enrichment analysis of miRNA target genes highlighted pathways related to ribonucleoprotein complex biogenesis, RNA processing, and gene expression regulation. These findings establish the critical role of sperm sncRNAs as regulators of fertility and potential biomarkers in breeding soundness tests for the precision farming of livestock for global food security.
Yazarlar (6)
1
Mustafa Bodu
ORCID: 0000-0002-8432-7347
2
Mustafa Hitit
3
Huseyin Donmez
4
Abdullah Kaya
5
Muhammet Rasit Ugur
6
Erdoğan Memili
Anahtar Kelimeler
fertility
miRNA
ram
small non-coding RNAs
sperm
Kurumlar
Faculty of Biology, Medicine and Health
Manchester United Kingdom
IVF Michigan Fertility Centers
Bloomfield United States
Kastamonu University
Kastamonu Turkey
Prairie View A&M University
Prairie View United States
Selçuk Üniversitesi
Selçuklu Turkey
University of Wisconsin-Madison
Madison United States