Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q2
Evaluation of the Cytotoxicity and Molecular Docking Properties of the Green Synthesis of Ag2S Nanoparticles
Journal of Cluster Science · Ağustos 2025
YÖKSİS Kayıtları
Evaluation of the Cytotoxicity and Molecular Docking Properties of the Green Synthesis of Ag2S Nanoparticles
Journal of Cluster Science · 2025 SCI-Expanded
Prof. Dr. MUSTAFA ÖZMEN →
Evaluation of the Cytotoxicity and Molecular Docking Properties of the Green Synthesis of Ag2S Nanoparticles
Journal of Cluster Science · 2025 SCI-Expanded
Prof. Dr. SALİH ZEKİ BAŞ →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Effect of Silver-Graphene Oxide-Cobalt Oxide Nanocomposite on Cytotoxic Levels in MRC-5 and HepG2 Cell Lines and Molecular Docking Studies
2024 ISSN: 1040-7278 SCI-Expanded Q2
Prof. Dr. MUSTAFA ÖZMEN →
Effect of Silver-Graphene Oxide-Cobalt Oxide Nanocomposite on Cytotoxic Levels in MRC-5 and HepG2 Cell Lines and Molecular Docking Studies
2024 ISSN: 1040-7278 SCI-Expanded Q2
Prof. Dr. SALİH ZEKİ BAŞ →
Evaluation of the Cytotoxicity and Molecular Docking Properties of the Green Synthesis of Ag2S Nanoparticles
2025 ISSN: 1040-7278 SCI-Expanded Q1
Prof. Dr. MUSTAFA ÖZMEN →
Makale Bilgileri
ISSN10407278
Yayın TarihiAğustos 2025
Cilt / Sayfa36
Scopus ID2-s2.0-105007072034
Erişim🔓 Açık Erişim
Özet
Pancreatic cancer is a type of cancer that is aggressive and has a high mortality rate with a five-year survival rate. The primary treatment option commonly used today is chemotherapy, which has very severe side effects. Considering all these side effects, the search for a new treatment for pancreatic cancer is extremely important. In this study, the cytotoxicity effect of silver sulfide nanoparticles (Ag<inf>2</inf>S NPs) synthesized with sweet cherry (Prunus avium L.), gemcitabine (GEM), a chemotherapy drug, and their combination (Ag<inf>2</inf>S NPs—GEM) on PANC-1 cancer cell lines was evaluated by MTT method. Additionally, the interaction of Ag<inf>2</inf>S NPs, GEM, and their combination with DNA was examined by conducting agarose gel electrophoresis. Also, interactions of Ag<inf>2</inf>S NPs, GEM, and their combination (Ag<inf>2</inf>S NPs—GEM) with key enzymes involved in nucleotide synthesis and DNA replication were investigated using molecular docking. The cytotoxicity results in PANC-1 cancer cell lines indicated that the most effective incubation period for Ag<inf>2</inf>S NPs was 24 hours (IC<inf>50</inf>: 75.77 µg/mL), while the most effective results for gemcitabine were obtained at 72 hours (IC<inf>50</inf>: 32.77 µg/mL). The combined application of Ag<inf>2</inf>S NPs and GEM produced significantly more effective results compared to monotherapy (IC<inf>50</inf>: 18.97 µg/mL in 72 h). DNA cleavage study showed that both Ag<inf>2</inf>S NPs and GEM bind to DNA, especially Ag<inf>2</inf>S NPs has affinity for G-G bases. The Ag<inf>2</inf>S NPs—GEM combination showed highly favorable binding affinities compared to Ag<inf>2</inf>S and metabolites of GEM, particularly against thymidylate synthase (ΔG = −6.52 kcal/mol) and DNA polymerase alpha catalytic core (ΔG = −6.65 kcal/mol), correlating with its potent cytotoxicity in PANC-1 cells. Additionally, docking simulations with B-DNA revealed that Ag<inf>2</inf>S NPs—GEM exhibits the strongest DNA-binding affinity (ΔG = −7.93 kcal/mol), acting as an effective DNA binder that may enhance cytotoxicity in PANC-1 cells.
Yazarlar (6)
1
Emine Arslan
2
Busra Ozcay Eksi
3
Erman Salih Istifli
4
Keziban Atacan
5
Salih Zeki Bas
ORCID: 0000-0003-2822-8851
6
Mustafa Ozmen
ORCID: 0000-0001-5117-9168
Anahtar Kelimeler
Ag2S
Cytotoxicity
DNA cleavage
Green synthesis
Molecular docking
Kurumlar
Çukurova Üniversitesi
Adana Turkey
Sakarya University of Applied Sciences
Serdivan Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Journal of Cluster Science
Q2
SJR Skoru0,552
H-Index74
YayıncıSpringer New York
ÜlkeUnited States
Chemistry (miscellaneous) (Q2)
Condensed Matter Physics (Q2)
Materials Science (miscellaneous) (Q2)
Biochemistry (Q3)
Metrikler
2
Atıf
6
Yazar
5
Anahtar Kelime