Scopus
YÖKSİS DOI Eşleşti
SJR Q1
Towards multimodal HPLC separations on humic acid-bonded aminopropyl silica: RPLC and LEC behavior
Talanta · Ekim 2011
YÖKSİS Kayıtları
Towards multimodal HPLC separations on humic acid bonded aminopropyl silica RPLC and LEC behavior
TALANTA · 2011 SCI-Expanded
Prof. Dr. HÜSEYİN KARA →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Square wave voltammetry based on determination of copper II ions by polyluteolin and polykaempferol modified electrodes
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4 tert butylcalix 4 arene having nitrile pendant groups as Hg2 selective receptors
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4 tert butylcalix 4 arene having nitrile pendant groups as Hg2 selective receptors
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Application of multivariate chemometric techniques for simultaneous determination of five parameters of cottonseed oil by single bounce attenuated total reflectance Fourier transform infrared spectroscopy
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Square wave voltammetry based on determination of copper II ions by polyluteolin and polykaempferol modified electrodes
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Development of poly 3 aminophenylboronic acid modified graphite rod electrode suitable for fluoride determination
2014 ISSN: 00399140 SCI-Expanded
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Development of a pH sensing membrane electrode based on a new calix 4 arene derivative
2015 ISSN: 00399140 SCI-Expanded 3 atıf
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Application of multivariate chemometric techniques for simultaneous determination of five parameters of cottonseed oil by single bounce attenuated total reflectance Fourier transform infrared spectroscopy
2014 ISSN: 0039-9140 SCI-Expanded
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Anthracene excimer based turn on fluorescent sensor for Cr3 and Fe3 ions Its application to living cells
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Novel magnetite nanoparticle based on BODIPY as fluorescent hybrid material for Ag I detection in aqueous medium
2016 ISSN: 00399140 SCI-Expanded
Prof. Dr. ERSİN GÜLER →
Anthracene excimer based turn on fluorescent sensor for Cr3 and Fe3 ions Its application to living cells
2016 ISSN: 00399140 SCI-Expanded
Prof. Dr. ÖZCAN KOÇYİĞİT →
Novel magnetite nanoparticle based on BODIPY as fluorescent hybrid material for Ag I detection in aqueous medium
2016 ISSN: 00399140 SCI-Expanded
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Rapid and real-time detection of arginine enantiomers by QCM sensorhaving a Calix[4]arene receptor bearing asymmetric centers
2019 ISSN: 0039-9140 SCI-Expanded
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Application of multivariate chemometric techniques for simultaneous determination of five parameters of cottonseed oil by single bounce attenuated total reflectance Fourier transform infrared spectroscopy
2014 ISSN: 0039-9140 SCI-Expanded
Prof. Dr. HÜSEYİN KARA →
Towards multimodal HPLC separations on humic acid bonded aminopropyl silica RPLC and LEC behavior
2011 ISSN: 0039-9140 SCI-Expanded
Prof. Dr. HÜSEYİN KARA →
Towards multimodal HPLC separations on humic acid bonded aminopropyl silica RPLC and HILIC behavior
2011 ISSN: 0039-9140 SCI-Expanded
Prof. Dr. HÜSEYİN KARA →
Makale Bilgileri
Dergi
Talanta
ISSN00399140
Yayın TarihiEkim 2011
Cilt / Sayfa85 · 2405-2410
Scopus ID2-s2.0-80053563013
Özet
In the present study, metal binding property of humic acid (HA) was successfully adapted to the ligand-exchange concept, and metal-loaded immobilized humic acid was used as a ligand exchanger stationary phase for separation of some nucleosides. Humic-acid bonded aminopropyl silica (EC-HA-APS) was turned into ligand exchanger forms by loading aqueous solutions of Cu 2+, and Co2+ to the column (4.6 × 150; as mm) packed with EC-HA-APS. Metal ion solutions were loaded to the column in a stepwise manner where the concentration of metal ion solution being loaded to the column was increased gradually between 5 and 100 mM. The progress of metal loading process was monitored via the breakthrough curves propagated stepwise. Ligand-exchange chromatography (LEC) studies were performed on an HPLC system, and chromatographic behaviors of the studied nucleosides (i.e. uridine, Urd; thymidine, Tyd; cytidine, Cyd; adenosine, Ado; and guanosine, Guo) were investigated on Cu2+ and Co2+ loaded forms of the EC-HA-APS (Cu-EC-HA-APS and Co-EC-HA-APS). Effect of mobile phase composition, temperature, and the type of metal ion loaded to the column on the retentive behaviors of the compounds was studied, in detail. The studied solutes exhibited mixed-mode RPLC/LEC behavior on the stationary phase. Metal-loaded column (M-EC-HA-APS) was easily regenerated into its original form, EC-HA-APS, with 98 ± 2% metal recoveries, by using aqueous mixture of EDTA + NH3 at pH = 7.5. Thus, the stationary phase exhibited a high flexibility between RPLC and LEC modes. This property, also, made it possible to convert the stationary phase into various ligand exchanger forms by loading different metal ions. Hence, capacity and selectivity of the stationary phase towards the studied species was manipulated easily by loading different metal ions to the stationary phase. Baseline separation for the studied species was achieved on Cu-EC-HA-APS and Co-EC-HA-APS and some differentiations were observed in capacity and selectivity, depending on the type of metal loaded. Thus, being as the first endeavor on usability of immobilized HA as a ligand exchanger stationary phase, the present study is believed to be useful to understand multifunctional character of HA-based solid/stationary phases. © 2011 Elsevier B.V. All rights reserved.
Yazarlar (2)
1
Orhan Gezici
2
Huseyin Kara
Anahtar Kelimeler
Humic acid
Immobilization
Ligand-exchange chromatography
Nucleosides
Separation
Stationary phase
Kurumlar
Niğde Ömer Halisdemir University
Nigde Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Talanta
Q1
SJR Skoru0,975
H-Index202
YayıncıElsevier B.V.
ÜlkeNetherlands
Analytical Chemistry (Q1)
Spectroscopy (Q1)
Biochemistry (Q2)
Metrikler
10
Atıf
2
Yazar
6
Anahtar Kelime