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Gut Microbiome and Tissue Morphology Modulatory Effects of Hazelnut (Natural, Roasted, and Skin) Fibers in Different Colonic Segments of Mice

Efood · Şubat 2025

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YÖKSİS Kayıtları
Gut Microbiome and Tissue Morphology Modulatory Effects of Hazelnut (Natural, Roasted, and Skin) Fibers in Different Colonic Segments of Mice
eFood · 2025
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Makale Bilgileri

DergiEfood
Yayın TarihiŞubat 2025
Cilt / Sayfa6
Erişim🔓 Açık Erişim
Özet To reveal the functional properties of hazelnut dietary fibers (DFs) in different colonic segments (cecum, proximal, and distal colon), a diet enriched with natural hazelnut, roasted hazelnut, or hazelnut skin DFs was applied to mice for 6 weeks; microbial metabolites, microbial composition, and tissue morphology were determined segmentally using gas chromatography, 16S rRNA sequencing technology, and microscopy, respectively. Roasted hazelnut DFs revealed significantly (p < 0.05) higher propionate in the cecum of female mice, while hazelnut skin DFs significantly increased the butyrate level in the distal colon of male counterparts. 16S rRNA sequencing revealed hazelnut DFs promoted the Lactobacillus animalis, L. gasseri, and Akkermansia muciniphila related OTUs, especially in the proximal colon, but the degrees of promotions were hazelnut type-, segment- and sex-dependent. Interestingly, hazelnut skin DFs significantly (p < 0.05) stimulated Prevotella related OTUs in the distal colon regardless of sex, which is known to have great ability to utilize dietary polysaccharides. Furthermore, hazelnut skin DF group had higher crypt height values, suggesting that hazelnut skin DFs have ability to maintain saccharolytic activity in more distal region of the colon. Overall, our results demonstrate that hazelnut DFs differentially impact microbial metabolite formation, microbiota composition and tissue morphology in different segments of the colon.

Yazarlar (12)

1
Ömer F. Çelik
ORCID: 0000-0002-6105-4825
2
Elanur Daştan
ORCID: 0000-0002-1613-6469
3
Ömer F. Çetiner
ORCID: 0000-0002-5132-5353
4
Orhan Baş
ORCID: 0000-0002-7449-2699
5
Zafer Bulut
6
Bin Zhang
ORCID: 0000-0003-4465-4826
7
Stephen R. Lindemann
ORCID: 0000-0002-3788-5389
8
Mehmet Tugay
ORCID: 0000-0001-7198-7744
9
Muhammet Değermenci
ORCID: 0000-0002-4751-6202
10
Beyza Suvarikli Alan
ORCID: 0000-0003-4698-9291
11
Mehmet Nizamlioǧlu
12
Yunus E. Tunçil
ORCID: 0000-0002-9421-2332

Anahtar Kelimeler

16S rRNA sequencing cecum Corylus avellana L. distal colon proximal colon short-chain fatty acids

Kurumlar

College of Agriculture
West Lafayette United States
College of Health and Human Sciences
West Lafayette United States
College of Science
West Lafayette United States
Dokuz Eylül Üniversitesi
Izmir Turkey
İstanbul Tıp Fakültesi
Istanbul Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Ordu Üniversitesi
Ordu Turkey
Samsun University
Samsun Turkey
Selçuk Üniversitesi
Selçuklu Turkey
South China University of Technology
Guangzhou China

Metrikler

1
Atıf
12
Yazar
6
Anahtar Kelime