Scopus
YÖKSİS Eşleşti
Bioactive Compounds of Plantago arenaria and Their Effects on SH-SY5Y Cells: Phenolic Content and Docking Analysis
Chemistry Africa · Kasım 2024
YÖKSİS Kayıtları
Bioactive Compounds of Plantago arenaria and Their Efects on SH‑SY5Y Cells: Phenolic Content and Docking Analysis
Chemistry Africa, Springer · 2024 ESCI
DOKTOR ÖĞRETİM ÜYESİ GAMZE DEMİREL →
Makale Bilgileri
DergiChemistry Africa
Yayın TarihiKasım 2024
Cilt / Sayfa7 · 4741-4755
Scopus ID2-s2.0-85207319471
Özet
This study investigated the antioxidant activity, phenolic content, and cytotoxic effects of Plantago arenaria extracts, alongside the identification of bioactive compounds and their interactions with Crystal structure of human PHGDH in complex with Homoharringtonine (PHGDH). Antioxidant assays revealed significant activity, with a DPPH IC<inf>50</inf> value of 266.21 ± 5.44 µg/mL and iron chelating capacity IC<inf>50</inf> of 4.22 ± 0.47 mg/mL. The total phenolic and flavonoid contents of the extract were measured at 194.25 ± 34.72 mg GAE/g and 51.47 ± 1.28 mg QE/g, respectively. Phytochemical analysis identified 12 bioactive compounds, with Anisole, o-(1-ethylvinyl)- having the highest percentage at 27.58%. Cytotoxicity studies using SH-SY5Y human neuroblastoma cells showed a 71 ± 2.5% reduction in viability at the highest extract dose, while normal HEK293 cells exhibited significantly increased proliferation, indicating selective cytotoxicity towards cancer cells. Molecular docking studies with PHGDH (7EWH) showed significant binding interactions for several compounds, with 2-Acetylcyclopentanone displaying the highest binding energy of − 4.4 kcal/mol. Toxicity profiling revealed varying degrees of toxicity across different parameters, with Acetic acid, propyl ester, and Anisole, o-(1-ethylvinyl)- being active in cardiotoxicity and carcinogenicity. These findings suggest that P. arenaria extracts possess strong antioxidant properties, significant phenolic and flavonoid content, selective cytotoxicity against cancer cells, and potential for developing PHGDH-targeted cancer therapies.
Yazarlar (6)
1
Zeynep Betül Sarı
2
Muhammet Emin Sarı
3
Emine Incilay Torunoğlu
ORCID: 0000-0003-4641-0067
4
Gamze Demirel
5
Betül Aydın
6
Erdi Can Aytar
ORCID: 0000-0001-6045-0183
Anahtar Kelimeler
Antioxidant activity
GC–MS analysis
Human neuroblastoma cells
Molecular docking
Plantago arenaria
Kurumlar
Ankara Yildirim Beyazit University
Ankara Turkey
Gazi Üniversitesi
Ankara Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Usak University
Usak Turkey
Metrikler
5
Atıf
6
Yazar
5
Anahtar Kelime