Scopus
YÖKSİS Eşleşti
Flow Cytometry and Gene Expression Modulation by Euphorbia rigida Methanol Extract in A549 Lung Cancer Cells: Induction of Apoptosis Through Bax, Caspase-9, and Bcl-2 Pathways
Advanced Biology · Ocak 2025
YÖKSİS Kayıtları
Flow Cytometry and Gene Expression Modulation by Euphorbia rigida Methanol Extract in A549 Lung Cancer Cells: Induction of Apoptosis Through Bax, Caspase-9, and Bcl-2 Pathways
Advanced Biology · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ GAMZE DEMİREL →
Makale Bilgileri
DergiAdvanced Biology
Yayın TarihiOcak 2025
Scopus ID2-s2.0-105006688448
Özet
Non-small cell lung cancer (NSCLC) remains a major cause of cancer-related mortality. This study investigates the cytotoxic effects of Euphorbia rigida extract on A549 NSCLC cells and its potential as a therapeutic agent. Cellular morphology was observed microscopically, and cell viability was evaluated using dose-dependent proliferation assays. Apoptosis-related gene expression—including Bax, Bcl-2, and Caspase-9—was analyzed via quantitative PCR (qPCR). Chromatographic methods identified bioactive flavonoids, and molecular docking assessed their binding to cancer-related proteins. Additionally, absorption, distribution, metabolism, excretion, and toxicity (ADMET) profiles were evaluated. The extract induced apoptotic morphological changes such as cell shrinkage and loss of intercellular contact. A dose-dependent reduction in A549 viability was observed, with an IC<inf>50</inf> of 0.5 mg mL<sup>−1</sup>. Gene expression indicated activation of the intrinsic mitochondrial apoptotic pathway, with increased Bax and Caspase-9 and decreased Bcl-2 expression. Flow cytometry using Annexin V-allophycocyanin (V-APC) staining revealed selective cytotoxicity: significant apoptosis in A549 cells while preserving viability in BEAS-2B normal lung epithelial cells. Identified flavonoids included quercetin, apigenin, and myricetin, which showed strong binding affinities in docking studies. ADMET profiling supported their drug-likeness. These findings highlight E. rigida potential in NSCLC treatment via apoptosis induction and selective cytotoxicity.
Yazarlar (8)
1
Zeynep Betül Sarı
2
Muhammed Emin Sarı
3
Erdi Can Aytar
ORCID: 0000-0001-6045-0183
4
Abidin Gümrükçüoğlu
5
Emine Incilay Torunoğlu
ORCID: 0000-0003-4641-0067
6
Tuba Ozdemir-Sanci
7
Gamze Demirel
8
Alper Durmaz
Anahtar Kelimeler
A549 cells
Bax
Bcl-2
BEAS-2B
Caspase-9
cytotoxicity
Euphorbia rigida
molecular docking
Kurumlar
Ankara Yildirim Beyazit University
Ankara Turkey
Artvin Coruh University, Turkey
Artvin Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Usak University
Usak Turkey