Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Regulatory Effects of Betonica macrantha Extract, Known as Mountain Tea, on miRNA Expression and Its Pharmacokinetic Properties
Food Science and Nutrition · Haziran 2025
YÖKSİS Kayıtları
Regulatory Effects of Betonica macrantha Extract,Known as Mountain Tea, on miRNA Expression and ItsPharmacokinetic Properties
Food Science & Nutrition · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ GAMZE DEMİREL →
Makale Bilgileri
DergiFood Science and Nutrition
Yayın TarihiHaziran 2025
Cilt / Sayfa13
Scopus ID2-s2.0-105006485852
Erişim🔓 Açık Erişim
Özet
The aim of this study is to investigate the anticancer potential of Betonica macrantha extract on MDA-MB-231 breast cancer cells, its regulatory effects on miRNA expression, the content analysis of its phytochemical components, and the roles of these compounds in the regulation of miRNA expression through pathways, as well as to examine its pharmacokinetic profiles. The B. macrantha plant was extracted with methanol. The obtained extracts were analyzed for phytochemical components using gas chromatography–mass spectrometry (GC–MS) and high-performance liquid chromatography (HPLC-DAD) techniques. Biological activity was assessed using the MTT assay on the MDA-MB-231 breast cancer cell line, while miRNA expression was measured by RT-PCR. Pharmacokinetic properties were calculated using ADMETSAR3 software, and molecular interactions were investigated through AutoDock Vina simulations. GC–MS analysis of B. macrantha extract identified 42 volatile compounds, with 1S-α-pinene, humulene, and caryophyllene being the most abundant. HPLC-DAD analysis detected 18 bioactive compounds, including catechin, oleuropein, and rutin. The extract inhibited the viability of MDA-MB-231 breast cancer cells in a dose-dependent manner, with an IC<inf>50</inf> value of 0.8 mg/mL. Furthermore, upregulation of miR-19, miR-20a, miR-126, and miR-200c miRNAs was observed in MDA-MB-231 cells, while these miRNAs were downregulated in healthy cells. ADMET analysis revealed that α-pinene, caryophyllene, and catechin exhibited high bioavailability, absorption, and distribution properties, while oleuropein and rutin showed limited absorption and bioavailability. Molecular docking studies demonstrated the potential binding interactions of these compounds with key target proteins involved in cancer progression. Consequently, B. macrantha presents significant potential as a valuable natural source for cancer therapy through its anticancer activity, modulation of miRNA expression, and interaction with cancer-associated proteins.
Yazarlar (7)
1
Muhammed Emin Sarı
2
Emine Incilay Torunoğlu
ORCID: 0000-0003-4641-0067
3
Zeynep Betül Sarı
4
Erdi Can Aytar
ORCID: 0000-0001-6045-0183
5
Abidin Gümrükçüoğlu
6
Alper Durmaz
7
Gamze Demirel
Anahtar Kelimeler
B. macrantha
GC–MS analysis
HPLC-DAD analysis
MDA-MB-231 cancer cell line
miRNA expression
molecular docking
Kurumlar
Ankara Yildirim Beyazit University
Ankara Turkey
Artvin Coruh University, Turkey
Artvin Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Usak University
Usak Turkey