Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Sperm cellular and nuclear dynamics associated with ram fertility
Frontiers in Veterinary Science · Ocak 2025
YÖKSİS Kayıtları
Sperm cellular and nuclear dynamics associated with ram fertility
Frontiers in Veterinary Science · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Makale Bilgileri
DergiFrontiers in Veterinary Science
Yayın TarihiOcak 2025
Cilt / Sayfa12
Scopus ID2-s2.0-105007068447
Erişim🔓 Açık Erişim
Özet
The aim of this study is to analyze DNA integrity, shape morphology, and membrane integrity in sperm from low-fertility (LF) and adequate- or normal-fertility (AF) rams. Various sperm evaluation methods such as sperm chromatin dispersion assay, Fourier harmonic amplitude (FHA) analysis, and other image analysis of morphometric parameters were used. An additional aim is to employ new statistical models with high reliability to predict ram fertility based on sperm head morphology parameters. Fresh semen was collected from 41 AF (conception rate 95.1% ± 0.6%) and 27 LF (conception rate 79.7% ± 2.5%) rams using artificial vagina. Differences (p < 0.05) were observed in percent motile sperm (mean ± SEM, 64% ± 3%, 72% ± 2%), percent viable sperm (78 ± 2%, 84 ± 1%), and head and acrosome abnormalities (1.9% ± 0.4%, 3.4% ± 0.4%) between LF and AF rams. The findings of different analyses also showed that the fertility of rams is not associated with DNA fragmentation (p > 0.05). Using the FHA analysis, an average head shape of ram sperm was constructed and harmonic amplitude 2 was determined, which tended to differ between the two ram fertility groups (p = 0.059). Based on the FHA and morphometric analysis, a significant linear discriminant model was constructed (p = 0.0013), which allowed for specificity in identifying LF rams (6/9, 66.7%) and sensitivity in identifying AF rams (39/47, 83.0%). The overall error rate remained good, which was 11/56 (20%). The findings of this study suggest that sperm DNA damage might not be used to predict ram fertility and that the statistical model based on the FHA analysis can be a potential tool in predicting ram fertility.
Yazarlar (10)
1
Mustafa Bodu
ORCID: 0000-0002-8432-7347
2
Mustafa Hitit
3
Ayşe Sarı
ORCID: 0000-0003-4181-9509
4
Mesut Kirbaş
5
Bülent Bülbül
6
M. B. Ataman
7
Mustafa Numan Bucak
8
John Parrish
9
Abdullah Kaya
10
Erdoğan Memili
Anahtar Kelimeler
DNA integrity
fertility
Fourier analysis
ram
sperm head
Kurumlar
Bahri Dagdas International Agricultural Research Institute
Konya Turkey
Dokuz Eylül Üniversitesi
Izmir Turkey
Kastamonu University
Kastamonu Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Prairie View A&M University
Prairie View United States
Selçuk Üniversitesi
Selçuklu Turkey
University of Wisconsin-Madison
Madison United States