Scopus
YÖKSİS Eşleşti
Method Validation for the Determination of Nicotine and Nicotine Metabolite Levels in Urine, Serum, and Saliva Samples
Biomedical Chromatography · Haziran 2025
YÖKSİS Kayıtları
Method Validation for the Determination of Nicotine and Nicotine Metabolite Levels in Urine, Serum, and Saliva Samples
Biomedical Chromatography · 2025 SCI-Expanded
PROFESÖR SEDAT ABUŞOĞLU →
Method Validation for the Determination of Nicotine and Nicotine Metabolite Levels in Urine, Serum and Saliva Samples
BIOMEDICAL CHROMATOGRAPHY · 2025 SCI-Expanded
PROFESÖR FATMA HÜMEYRA YERLİKAYA AYDEMİR →
Method Validation for the Determination of Nicotine and Nicotine Metabolite Levels in Urine, Serum, and Saliva Samples
Biomedical Chromatography · 2025 SCI-Expanded
PROFESÖR ALİ ÜNLÜ →
Makale Bilgileri
DergiBiomedical Chromatography
Yayın TarihiHaziran 2025
Cilt / Sayfa39
Scopus ID2-s2.0-105003952485
Özet
Monitoring nicotine and its metabolites in bodily fluids is crucial for assessing tobacco exposure and related toxicity in clinical practice. Due to its high sensitivity for detecting low-level analytes, liquid chromatography–tandem mass spectrometry (LC–MS/MS) was employed to enhance a robust and accurate analytical procedure for quantifying nicotine and its derivatives in serum, urine, and saliva. Comparative analysis was conducted on samples collected from individuals with and without smoking habits. The LC–MS/MS method utilized an API 3200 triple quadrupole instrument paired with a Phenomenex Luna C18 column. Method sensitivity was demonstrated through evaluation of sensitivity parameters, including LOD and LLOQ for each analyte in all matrices. Inter-assay variability and bias values at the LLOQ level were kept below 20%. In serum, the LOD–LLOQ ranges were 0.02–0.05 μg/L for nicotine, 0.32–0.95 μg/L for cotinine, 0.07–0.25 μg/L for 3-OH cotinine, and 0.22–0.69 μg/L for norcotinine. In urine, the respective values were 0.03–0.10, 0.23–0.82, 0.06–0.14, and 0.20–0.55 μg/L, and in saliva, the values were 0.12–1.59, 0.22–2.34, 0.10–0.17, and 0.33–3.01 μg/L. The proposed method is efficient, specific, and adaptable to routine clinical workflows, providing a valuable tool for monitoring tobacco-related exposure.
Yazarlar (5)
1
Gulsum Abusoglu
2
Duygu Eryavuz Onmaz
ORCID: 0000-0001-8564-1824
3
Ali Ünlü
ORCID: 0000-0002-9991-3939
4
Fatma Humeyra Yerlikaya Aydemir
ORCID: 0000-0002-4107-5389
5
Sedat Abusoglu
ORCID: 0000-0002-2984-0527
Anahtar Kelimeler
cotinine
LC MS/MS
method validation
nicotine
Kurumlar
Bandırma Onyedi Eylül University
Bandirma Turkey
Selçuk Tip Fakültesi
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey