Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Virulence genes of Pasteurella multocida cap B and its potential cross protection in mice
International Microbiology · Ocak 2025
YÖKSİS Kayıtları
Virulence genes of Pasteurella multocida cap B and its potential cross protection in mice
International Microbiology · 2025 SCI-Expanded
DOÇENT ASLI BALEVİ →
Makale Bilgileri
DergiInternational Microbiology
Yayın TarihiOcak 2025
Scopus ID2-s2.0-105003965671
Erişim🔓 Açık Erişim
Özet
Pasteurella multocida is a Gram-negative coccobacillus from the Pasteurellaceae family, commonly residing as a commensal organism in the respiratory tracts of healthy animals. However, it possesses multiple virulence factors and can cause severe respiratory diseases. This study aimed to characterize P. multocida and its virulence genes and evaluate the immunogenicity of an inactivated vaccine of serogroup B using different administration routes. A total of 250 samples were collected from animals showing respiratory symptoms. Using 5% blood agar, 27 P. multocida isolates were obtained, and 21 (8.4%) were confirmed via PCR targeting the kmt1 gene. Nineteen virulence-associated genes were screened, categorized into outer membrane, fimbrial, somatic antigen, and iron-binding genes. The plpB, tadD, gatG, and hgbA genes were detected in both serogroup B and E isolates, whereas ompA, toxA, pcgD, latB, nctB, ppgB, natG, hgbB, and exbB were absent in all isolates. The immunogenicity of an inactivated P. multocida vaccine was evaluated in mice using subcutaneous and intramuscular routes. Subcutaneous vaccination produced a significantly higher antibody titer at 3 and 5 weeks post-vaccination with a 0.5 mL dose; in contrast, intramuscular immunization resulted in a rapid increase after booster doses. Analysis of variance (ANOVA) and Duncan’s multiple range test (p < 0.05) revealed statistically significant differences between treatments. The comparison between subcutaneous and intramuscular routes also showed a significant difference (p < 0.001). This study concludes that although P. multocida serogroup B harbors fewer virulence factors, it effectively induces an immune response in mice but fails to provide cross-protection against the local serogroup E strain.
Yazarlar (3)
1
Adam Bashir Tawor
2
O. Erganiş
3
Aslı Balevı
Anahtar Kelimeler
Cross-protection
Immune response
Respiratory infection
Virulence genes Pasteurella multocida
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
University of Al Gadarif
Al-Gadarif Sudan