Scopus
YÖKSİS Eşleşti
Comparative Analysis of Neospora caninum Isolation Success from Various Fetal Tissues: The Importance of Brain Samples. Technical note
Revista Cientifica De La Facultad De Veterinaria · Ocak 2025
YÖKSİS Kayıtları
Comparative Analysis of Neospora caninum Isolation Success from Various Fetal Tissues: The Importance of Brain Samples. Technical note
Revista Científica de la Facultad de Ciencias Veterinarias · 2025 SCI-Expanded
DOÇENT ASLI BALEVİ →
Makale Bilgileri
DergiRevista Cientifica De La Facultad De Veterinaria
Yayın TarihiOcak 2025
Cilt / Sayfa35 · 1-7
Scopus ID2-s2.0-105005995622
Özet
Isolating Neospora caninum from naturally infected hosts is crucial for understanding the epidemiology of neosporosis. Although the parasite was reported to be isolated from various fetal tissues, including the brain, the relative successful isolation rates from different sources have not been systematically explored. This study examines the rates of isolating N. caninum from different fetal tissues, focusing on the importance of brain samples. Fetal tissues from 90 naturally infected sheep were analyzed for the presence of N. caninum. Samples were processed for molecular and cell culture isolation techniques. Tachyzoite yields and cytopathogenic effects (CPE) were compared across tissues following isolation in Vero cells. Of the 90 samples, 19 (21.1%) were positive for N. caninum by PCR, and 8 (42.1%) of these yielded viable isolates. Sheep brain tissue exhibited significantly higher tachyzoite yields (1.9 x 107) compared to other tissues (abomasal contents, kidney, placenta, liver and pericardial fluid). The greatest CPE effect at 80% was observed in the eighth passage. Brain samples appear to be the most reliable source for in vitro isolation of N. caninum from naturally infected fetuses. This finding has important implications for the diagnosis and study of sheep neosporosis.
Yazarlar (8)
1
Aslı Balevı
2
Beatriz Padron
3
Aysegul Ilban
ORCID: 0000-0001-7450-551X
4
Figen Celik
5
Emine Eda Toslak
6
O. Denizli
7
O. Erganiş
8
Sami Simsek
Anahtar Kelimeler
Abortion
Aborto
aislamiento de cultivo celular
brain
cerebro
Neospora caninum
ovejas
sheep
tissue culture isolation
Kurumlar
Firat Üniversitesi
Elazig Turkey
Ordu Üniversitesi
Ordu Turkey
Selçuk Üniversitesi
Selçuklu Turkey