Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D
Turkish Journal of Biochemistry · Aralık 2022
YÖKSİS Kayıtları
Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D
Turkish Journal of Biochemistry · 2022 SCI-Expanded
PROFESÖR ALİ ÜNLÜ →
Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D
Turkish Journal Of Biochemistry · 2022 EBSCO, İndex Copernicus
PROFESÖR ALİ ÜNLÜ →
Makale Bilgileri
DergiTurkish Journal of Biochemistry
Yayın TarihiAralık 2022
Cilt / Sayfa47 · 710-718
Scopus ID2-s2.0-85141192959
Erişim🔓 Açık Erişim
Özet
Objectives: Vitamin D is one of the major hormones involved in the metabolism of calcium (Ca) and phosphorus (P). In the present study, we aimed to determine the analytical performance of the immunoassay method used for determining plasma 25-hydroxyvitamin D [25(OH)D] levels in routine clinical practice in laboratories. Methods: Venous blood samples were collected from 156 patients for the comparisons and were analyzed with Siemens ADVIA Centaur XPT, the Roche Cobas 6,000's module e601, Abbott Architect i2000, and the liquid chromatography with tandem mass spectrometry (LC-MS/MS). Results: The four methods were analyzed and compared through the Passing-Bablok regression for 25(OH)D, and the highest correlation was found at LC-MS/MS and Cobas 6,000's module e601 (r=0.799), LC-MS/MS/Abbott Architect i2000, and LC-MS/MS/Siemens ADVIA Centaur XPT as r=0.736, 0.721, respectively. The correlation coefficient was found between Abbott Architect i2000 with Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.934 and r=0.907, respectively. Also, the correlation coefficient was found between Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.906. Conclusions: The Roche Cobas assay showed better performance, compared with the other assays. Based on our findings, the chemiluminescence methods in automated systems seem to be expedient.
Yazarlar (5)
1
Saadet Kader
2
Turan Akdağ
ORCID: 0000-0003-3175-6751
3
Busra Ecer
4
Sedat Abusoglu
ORCID: 0000-0002-2984-0527
5
Ali Ünlü
ORCID: 0000-0002-9991-3939
Anahtar Kelimeler
25 (OH)D
assay
chemiluminescence
immunoassay methods
liquid chromatography with tandem mass spectrometry
Kurumlar
Karaplnar State Hospital
Konya Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Tip Fakültesi
Konya Turkey
Metrikler
1
Atıf
5
Yazar
5
Anahtar Kelime