Scopus
YÖKSİS Eşleşti
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · Aralık 2024
YÖKSİS Kayıtları
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 SCI-Expanded
DOÇENT DUDU ERKOÇ KAYA →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 SCI-Expanded
PROFESÖR ESMA MENEVŞE →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ DUYGU DURSUNOĞLU →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 EBSCO
PROFESÖR HİLAL ARIKOĞLU →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 EBSCO
DOKTOR ÖĞRETİM ÜYESİ DUYGU DURSUNOĞLU →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ DUYGU DURSUNOĞLU →
Makale Bilgileri
DergiMolecular Biology Reports
Yayın TarihiAralık 2024
Cilt / Sayfa51
Scopus ID2-s2.0-85185861030
Özet
Background: Treatment of Pancreatic Cancer (PC) is challenging due to its aggressiveness and acquired resistance to conventional chemotherapy and radiotherapy. Therefore, the discovery of new therapeutic agents and strategies is essential. Juglone, a naphthoquinone, is a secondary metabolite produced naturally in walnut-type trees having allelopathic features in its native environment. Juglone was shown to prevent cell proliferation and induce ROS-mediated mitochondrial apoptosis. Ascorbate with both antioxidant and oxidant features, shows selective cytotoxicity in cancer cells. Methods and results: In this study, we evaluated the anticancer effects of Juglone in combination with ascorbate in PANC-1 and BxPC-3 PC cells. The MTT assay was used to determine the IC50 dose of Juglone with 1 mM NaAscorbate (Jug-NaAsc). Subsequently, the cells were treated with 5, 10, 15 and 20 µM Jug-NaAsc for 24 h. Apoptotic effects were evaluated by analyzing the following genes using qPCR; proapoptotic Bax, antiapoptotic Bcl-2 related to the mitochondrial apoptotic pathway and apoptosis inhibitor Birc5 (Survivin). Immunofluorescence analysis was performed using Annexin V-FITC in PC cells. As an antioxidant enzyme, Trx2 protein levels were determined by a commercial ELISA test kit. Jug-NaAsc treatment decreased the expressions of antiapoptotic genes Bcl-2 and Birc5 while the apoptotic gene Bax expression increased at all doses. Additionally, a dose-dependently increase of apoptosis according to immunofluorescence analysis and the decreases of Trx2 enzyme levels at all treatments in both cell lines supported gene expression results. Conclusion: Our results suggest that Juglone is a potential anticancer agent especially when combined with ascorbate.
Yazarlar (5)
1
Dudu Erkoc-Kaya
ORCID: 0000-0003-0114-6602
2
Hilal Arikoglu
ORCID: 0000-0002-6600-6603
3
Ebru Guclu
ORCID: 0000-0001-5330-6159
4
Duygu Dursunoǧlu
ORCID: 0000-0003-4414-8659
5
Esma Menevşe
Anahtar Kelimeler
Apoptosis
Juglone-Ascorbate
Pancreatic cancer
Prooxidant cancer therapy
Trx2
Kurumlar
Bozok Üniversitesi
Yozgat Turkey
Selçuk Tip Fakültesi
Konya Turkey
Metrikler
2
Atıf
5
Yazar
5
Anahtar Kelime