Scopus
YÖKSİS Eşleşti
SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)
Journal of Periodontal Research · Ağustos 2021
YÖKSİS Kayıtları
SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)
JOURNAL OF PERIODONTAL RESEARCH · 2021 SCI
PROFESÖR ERDOĞAN EŞREF HAKKI →
SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)
Journal of Periodontal Research · 2021 SCI-Expanded
PROFESÖR ALİ ÜNLÜ →
SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)
Journal of Periodontal Research · 2021 SCI-Expanded
PROFESÖR SEMA HAKKI →
Makale Bilgileri
DergiJournal of Periodontal Research
Yayın TarihiAğustos 2021
Cilt / Sayfa56 · 774-781
Scopus ID2-s2.0-85102633944
Özet
Background/Objectives: In this in vitro study, the effects of Stromal cell-derived factor-1 (SDF-1) was evaluated on the periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs) functions. Material and Methods: Real-time cell analyzer-single plate (RTCA-SP) was employed for proliferation, and RTCA-dual purpose (DP) was utilized for pdl-MSCs migration potential treated with different SDF-1 concentrations (0, 0.1, 1, 10, 100, 200, and 400 ng/ml). Based on the dose-response findings, 10 ng/ml SDF-1 was used for further mRNA experiments. RNAs isolated at 6 and 24 h were checked using quantitative RT-PCR for mineralized tissue-associated genes including type I collagen (COL I), osteocalcin (OCN), osteopontin (OPN), and runt-related transcription factor 2 (Runx2). cRNA was synthesized for 6 h, and whole-genome array analysis was performed for over 47.000 probes. Data were subjected to quantile normalization before analysis. Results: Increased proliferation and migration were observed in pdl-MSCs treated with 0.1, 1, and 10 ng/ml SDF-1. Increased COL I was observed at both time points: 6 and 24 h. While there was no significant change for OCN, OPN, and Runx2 at 6 h, SDF-1 up-regulated OCN and OPN, but down-regulated Runx2 mRNA expressions at 24 h. IL-8 and ESM1 genes were differentially expressed over twofold when the pdl-MSCs were exposed to SDF-1 at whole-genome array analysis. IL-8 induction was confirmed with RT-PCR. Conclusion: Findings of this study displayed that SDF-1 modulated pdl-MSCs which were important for periodontal regeneration, inducing migration and proliferation, and regulating extracellular matrix synthesis in favor of the formation of new attachment.
Yazarlar (6)
1
Sema S. Hakkı
2
Buket S. Bozkurt
3
E. Eşref Hakki
4
Erdal Karaoz
5
Ali Ünlü
ORCID: 0000-0002-9991-3939
6
Seyit Ali Kayis
Anahtar Kelimeler
IL-8
mRNA expression
periodontal ligament-Mesenchymal Stem Cells
real-time migration
real-time proliferation
stromal cell-derived factor-1
whole genome array
Kurumlar
Abant Izzet Baysal University, Faculty of Medicine
Bolu Turkey
Hacettepe Üniversitesi
Ankara Turkey
İstinye Üniversitesi
Istanbul Turkey
Liv Hospital
Istanbul Turkey
Selçuk Tip Fakültesi
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
5
Atıf
6
Yazar
7
Anahtar Kelime