Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Hyaluronic acid enhances cell migration, viability, and mineralized tissue-specific genes in cementoblasts
Journal of Periodontal Research · Şubat 2024
YÖKSİS Kayıtları
Hyaluronic acid enhances cell migration, viability, and mineralized tissue‐specific genes in cementoblasts
Wiley · 2024 SCI-Expanded
PROFESÖR SEMA HAKKI →
Makale Bilgileri
DergiJournal of Periodontal Research
Yayın TarihiŞubat 2024
Cilt / Sayfa59 · 63-73
Scopus ID2-s2.0-85178911655
Erişim🔓 Açık Erişim
Özet
Background/Objectives: It has been repeatedly demonstrated that cementum formation is a crucial step in periodontal regeneration. Hyaluronic acid (HA) is an important component of the extracellular matrix which regulates cells functions and cell–cell communication. Hyaluronic acid/derivatives have been used in regenerative periodontal therapy, but the cellular effects of HA are still unknown. To investigate the effects of HA on cementoblast functions, cell viability, migration, mineralization, differentiation, and mineralized tissue-associated genes and cementoblast-specific markers of the cementoblasts were tested. Materials and Methods: Cementoblasts (OCCM-30) were treated with various dilutions (0, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128) of HA and examined for cell viability, migration, mineralization, and gene expressions. The mRNA expressions of osteocalcin (OCN), runt-related transcription factor 2 (Runx2), bone sialoprotein (BSP), collagen type I (COL-I), alkaline phosphatase (ALP), cementum protein-1 (CEMP-1), cementum attachment protein (CAP), and small mothers against decapentaplegic (Smad) -1, 2, 3, 6, 7, β-catenin (Ctnnb1) were performed with real-time polymerase chain reaction (RT-PCR). Total RNA was isolated on days 3 and 8, and cell viability was determined using MTT assay on days 1 and 3. The cell mineralization was evaluated by von Kossa staining on day 8. Cell migration was assessed 2, 4, 6, and 24 hours following exposure to HA dilutions using an in vitro wound healing assay (0, 1:2, 1:4, 1:8). Results: At dilution of 1:2 to 1:128, HA importantly increased cell viability (p <.01). HA at a dilution of 1/2 increased wound healing rates after 4 h compared to the other dilutions and the untreated control group. Increased numbers of mineralized nodules were determined at dilutions of 1:2, 1:4, and 1:8 compared with control group. mRNA expressions of mineralized tissue marker including COL-I, BSP, RunX2, ALP, and OCN significantly improved by HA treatments compared with control group both on 3 days and on 8 days (p <.01). Smad 2, Smad 3, Smad 7, and β-catenin (Ctnnb1) mRNAs were up-regulated, while Smad1 and Smad 6 were not affected by HA administration. Additionally, HA at dilutions of 1:2, 1:4, and 1:8 remarkably enhanced CEMP-1 and CAP expressions in a dilution- and time-dependent manner (p <.01). Conclusions: The present results have demonstrated that HA affected the expression of both mineralized tissue markers and cementoblast-specific genes. Positive effects of HA on the cementoblast functions demonstrated that HA application may play a key role in cementum regeneration.
Yazarlar (4)
1
Sema S. Hakkı
2
Şerife Buket Bozkurt
3
Anton Sculean
4
Darko Božić
ORCID: 0000-0002-0391-0574
Anahtar Kelimeler
cell viability
cementoblast
hyaluronic acid
migration
mineralization
mRNA expression
Kurumlar
KBC Zagreb
Zagreb Croatia
Niğde Ömer Halisdemir University
Nigde Turkey
Selçuk Üniversitesi
Selçuklu Turkey
University of Bern, Faculty of Medicine
Bern Switzerland
Metrikler
8
Atıf
4
Yazar
6
Anahtar Kelime