Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Hypericum empetrifolium and H. lydium as Health Promoting Nutraceuticals: Assessing Their Role Combining In Vitro In Silico and Chemical Approaches
Food Science and Nutrition · Nisan 2025
YÖKSİS Kayıtları
Hypericum empetrifolium and H. lydium as Health Promoting Nutraceuticals: Assessing Their Role Combining In Vitro In Silico and Chemical Approaches
Food Science & Nutrition · 2025 SCI-Expanded
PROFESÖR EVREN YILDIZTUGAY →
Makale Bilgileri
DergiFood Science and Nutrition
Yayın TarihiNisan 2025
Cilt / Sayfa13
Scopus ID2-s2.0-105002072206
Erişim🔓 Açık Erişim
Özet
Hypericum species are known for their ability to produce multiple classes of secondary metabolite and in this article the possible health-promoting role of H. empetrifolium and H. lydium have been evaluated combining in vitro and in silico approaches. H. empetrifolium and H. lydium extracts were obtained using different solvents (ethyl acetate, aceton, aceton/water, and water) and the composition was compared using NMR and LC–MS based approaches. Myricetin-3-O-glucoside, Kaemempferol -3-O-glucoside, and hyperopliphylirrin were present only in H. empetrifolium. Rutin, quercetin-3-O-rhamnoside ant the triterpenoids oleanolic and ursolic acid were only detected in H. lydium. To establish a possible role against degenerative diseases antioxidant, antiradical activities of the extracts were studied and H. empetrifolium acetone/water and water extracts were the more active regarding these effects. The extracts were also evaluated for the inhibition of key-enzymes involved in degenerative diseases namely cholinesterase and tyrosinase for CNS-related pathologies and amylase for metabolic-related diseases. Significant inhibition of acetylcholinesterase was observed for the extracts obtained with lipophilic solvents. The extracts were also studied for their possible antiproliferative activity on cell lines including tumor (DU-145, A549, and MCF-7) and non-tumoral cells (HEK-293) revealing moderate activities. H. lydium ethyl acetate showed late apoptotic (17.5%) and necrotic (46.6%) effects in the Annexin V/PI assay. Molecular docking was used to establish possible interaction of identified compounds with target enzymes and a good interaction between rutin and tyrosinase, myricetin-7-O-glucoside and amylase, was reported. In conclusion, Hypericum empetrifolium and H. lydium thanks to their complex pattern of phytoconstituents and thanks to their significant antioxidant effects as well as with the ability to act on some key-target enzymes involved in degenerative diseases can be considered a good vegetal source for the preparation of nutraceuticals and food supplements useful as health-promoting products against oxidative stress-related diseases such as diabetes, cancer, and Alzheimer's disease.
Yazarlar (16)
1
Stefania Sut
2
Stefano Dall'Acqua
3
Giancarlo Angeles Flores
ORCID: 0000-0001-6651-9794
4
Gaia Cusumano
5
Ismail Koyuncu
6
Ozgur Yuksekdag
7
Carla Emiliani
8
Roberto Venanzoni
ORCID: 0000-0002-7768-0468
9
Paola Angelini
ORCID: 0000-0002-6862-1079
10
Selami Selvi
11
Evren Yildiztugay
ORCID: 0000-0002-4675-2027
12
Adriano Mollica
13
Eleonora Procino
14
Gregorio Peron
ORCID: 0000-0002-6007-6184
15
Lorenza Marinaccio
16
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
antioxidant
apoptosis
hyperforin
Hypericum
hyperoside
tyrosinase
Kurumlar
Balikesir Üniversitesi
Balikesir Turkey
Harran University, Faculty of Medicine
Sanliurfa Turkey
SCM Nutraceutici Universitari Srl
Chieti Italy
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Brescia
Brescia Italy
Università degli Studi di Padova
Padua Italy
Università degli Studi di Perugia
Perugia Italy
University of G. d'Annunzio Chieti and Pescara
Chieti Italy