Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q4
Molecular detection of leptospirosis from genital system in mares
Turk Hijyen Ve Deneysel Biyoloji Dergisi · Ocak 2024
YÖKSİS Kayıtları
Molecular detection of leptospirosis from genital system in mares
Turkish Bulletin of Hygiene and Experimental Biology · 2024 TR DİZİN
Doç. Dr. ASLI BALEVİ →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Effect of different selenium levels on thyroid hormone synthesis
2015 ISSN: 0377-9777 Index Copernicus
Prof. Dr. SEDAT ABUŞOĞLU →
Bir üniversite hastanesindeki yardımcı personelin hastaneenfeksiyonları ile ilgili bilgi ve tutumlarının değerlendirilmesi
2016 ISSN: 0377-9777 Index Copernicus
Dr. Öğr. Üyesi SELMA İNFAL KESİM →
Konya bölgesinde toplum kökenli pnömoni için hastaneye yatırılan çocuk hastalarda solunumsal viral enfeksiyon etkenlerinin multipleks gerçek zamanlı PCR ile tanımlanması
2012 ISSN: 0377-9777 Index copernicus, Ulakbim, Cross Ref, TÜRKİYE ATIF DİZİNİ
Prof. Dr. MELİKE EMİROĞLU →
Clinical characteristics and incidence of bacterial and viral pathogens in patients hospitalized with community acquired pneumonia in childhood in Konya between October 2008 and February 2010
2016 ISSN: 0377-9777 TR DİZİN
Prof. Dr. MELİKE EMİROĞLU →
Identification of respiratory viral infection agents by multiplex real-time PCR among children hospitalized for community-acquired pneumonia in Konya province
2012 ISSN: 0377-9777 TR DİZİN
Prof. Dr. MELİKE EMİROĞLU →
A pediatric case report of gastroenteritis caused by verocytotoxin producing Escherichia coli (VTEC) and enteric adenovirus
2011 ISSN: 0377-9777 TR DİZİN
Prof. Dr. MELİKE EMİROĞLU →
Periferik arter hastalarinda yükselmiş serum homositrüllin düzeyleri
2022 ISSN: 0377-9777 İndex copernicus
Prof. Dr. ABDULLAH SİVRİKAYA →
Influenza vaccination status in adults with chronic diseases between 2015-2017
2022 ISSN: 0377-9777 TR DİZİN
Prof. Dr. FATİH KARA →
Elevated serum homocitrulline levels in patients with peripheral artery disease
2022 ISSN: 0377-9777 İndex Copernicus
Prof. Dr. ABDULLAH SİVRİKAYA →
Embelin’in insan meme kanseri MCF-7 ve MDA MB-231 hücre proliferasyonu üzerindeki sitotoksik etkisinin tamoxifen ve docetaxel ile karşılaştırılması
2023 ISSN: 0377-9777 TR DİZİN
Prof. Dr. HÜSAMETTİN VATANSEV →
Comparison of the cytotoxic effect of Embelin with tamoxifen and docetaxel on human breast cancer MCF-7 and MDA MB-231 cell proliferation
2023 ISSN: 0377-9777 TR DİZİN
Prof. Dr. BAHADIR ÖZTÜRK →
Comparison of the cytotoxic effect of Embelin with tamoxifen and docetaxel on human breast cancer MCF-7 and MDA MB-231 cell proliferation
2023 ISSN: 0377-9777 TR DİZİN
Prof. Dr. HÜSAMETTİN VATANSEV →
Molecular detection of leptospirosis from genital system in mares
2024 ISSN: 0377-9777 TR DİZİN
Doç. Dr. ASLI BALEVİ →
Makale Bilgileri
ISSN03779777
Yayın TarihiOcak 2024
Cilt / Sayfa81 · 253-258
Scopus ID2-s2.0-85205810888
Erişim🔓 Açık Erişim
Özet
Objective: Leptospirosis is a worldwide zoonotic disease and well recognized infectious disease of horses. Equine leptospirosis is to cause the birth of weak foals, neonatal deaths and abortion after pregnancy period. Studies on leptospirosis in horses are generally investigations of urine samples and serological studies. Although leptospirosis causes reproductive disorders, genital sample studies that may be a source of infection have been ignored. The aims of this study were to study the prevalance of Leptospira by PCR using vaginal swab from apparently healthy horses. Methods: A total of 92 vaginal swab samples were collected and transferred to the bacterial diagnosis laboratory of Selcuk University Veterinary Faculty. The vulva and vagina were cleaned before swab samples were taken. All samples were stored in the refrigerator at -20°C and taken to the laboratory for processing. These samples were sent to the laboratory under cold chain conditions. DNA was extracted from suspicious samples and conventional PCR was used to detect Leptospira spp. Specific primers were selected and PCR was finalized for Leptospira spp. Results: As a result of the study, it was found that out of 92 mare’s vaginal swab samples 7 (7.6%) were positive for Leptospira spp. This study is the first report from our country due to the detection of Leptospira spp DNA in asymptomatic mares. Conclusion: It revealed that the Leptospira PCR positive mare were not showing any signs and symptoms. When the results and observations were evaluated, it was thought that Leptospira PCR positive mares could play a role as a carrier in the transmission of leptospirosis. Our study is one of the rare studies on mares carrying the possible causative agent. Detection of leptospirosis by PCR can be considered as a reliable method for early detection of Leptospira shedding in asymptomatic animals. In addition, molecular studies from vaginal swab samples were observed as a rapid and definitive diagnostic option, considering the difficulty of isolation of the Leptospira agent and possible contaminations. According to the results of our study, it is recommended to reevaluate the control measures against the disease and to carry out molecular characterization and vaccination studies in risky areas.
Yazarlar (3)
1
Derya Karataş Yeni
ORCID: 0000-0001-7261-1394
2
Aslı Balevı
3
A. Gok
Anahtar Kelimeler
kısrak
Leptospirosis
Leptospirozis
mare
PCR
Kurumlar
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Turk Hijyen ve Deneysel Biyoloji Dergisi
Q4
OA
SJR Skoru0,137
H-Index11
YayıncıRefik Saydam National Public Health Agency (RSNPHA)
ÜlkeTurkey
Infectious Diseases (Q4)
Microbiology (medical) (Q4)
Public Health, Environmental and Occupational Health (Q4)