Scopus
YÖKSİS Eşleşti
The neuroprotective effect of 1,25-dyhydroxyvitamin D3 (calcitriol) and probiotics on the rotenone-induced neurotoxicity model in SH-SY5Y cells
Drug and Chemical Toxicology · Ocak 2025
YÖKSİS Kayıtları
The neuroprotective effect of 1,25-dyhydroxyvitamin D3 (calcitriol) and probiotics on the rotenone-induced neurotoxicity model in SH-SY5Y cells
Drug and Chemical Toxicology · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ FATMA HAZAN DOĞAN →
Makale Bilgileri
DergiDrug and Chemical Toxicology
Yayın TarihiOcak 2025
Cilt / Sayfa48 · 72-83
Scopus ID2-s2.0-85210168358
Özet
This study aimed to investigate the neuroprotective role of probiotics and 1,25-dyhydroxyvitamin D3 (calcitriol) against neurotoxicity on rotenone-induced human neuroblastoma cell line SH-SY5Y. Rotenone was administered to induce neurotoxic effects in SH-SY5Y cells. Calcitriol and probiotics were administered at different concentrations as pre- and post-treatment. The thiazolyl blue tetrazolium bromide (MTT) assay was performed to measure cell viability. Intracellular protein levels of antioxidant enzymes (protein tyrosine kinase (PTK), superoxide dismutase (SOD), glutathione peroxidase (GSH), glutathione reductase (GSR), and catalase (CAT)) were determined by the enzyme-linked immunosorbent assay (ELISA). Rotenone (150 nM) reduced (p < 0.001) cell viability compared to control cells. Single and combined pretreatments with probiotics (0.01 mg/ml, 0.05 mg/ml, and 0.1 mg/ml) and calcitriol (1.25 µM, 2.5 µM, and 5 µM) increased (p < 0.05) cell viability compared to rotenone group. In the pre- and post-treatment design, all treatment groups increased the SOD and GSH levels and decreased the GSR levels compared to rotenone. None of the pretreatments reversed the PTK levels (except probiotics: 0.01 mg/ml). Calcitriol (2.5 µM) increased the CAT levels in pretreatment design, and probiotics (0.05 mg/ml and 0.1 mg/ml) increased CAT levels in post-treatment design compared to rotenone group. Calcitriol and probiotics protect against rotenone-induced neurotoxicity in SH-SY5Y cells by decreasing reactive oxygen species (ROS) and increasing antioxidant enzyme parameters. These neuroprotective effects of calcitriol and probiotics against rotenone-induced dopaminergic neurotoxicity provide an experimental basis for their potential clinical use in the treatment of Parkinson’s disease (PD).
Yazarlar (4)
1
Fatma Hazan Gul
ORCID: 0000-0003-2776-808X
2
Nuh Mehmet Bozkurt
ORCID: 0000-0003-2965-6719
3
Nalan Hakime Nogay
ORCID: 0000-0002-9435-5755
4
Gokhan Unal
Anahtar Kelimeler
1,25-dyhydroxyvitamin D 3
neuroprotective
neurotoxicity
Parkinson’s disease
probiotics
SH-SY5Y cell-line
Kurumlar
Bursa Uludağ Üniversitesi
Bursa Turkey
Erciyes Üniversitesi
Kayseri Turkey
Mersin Üniversitesi
Mersin Turkey
Metrikler
1
Atıf
4
Yazar
6
Anahtar Kelime