Scopus
YÖKSİS Eşleşti
Effect of serotonin 7 receptors and transcript variants on RIN-5F pancreatic beta cell line
Toxicology in Vitro · Haziran 2025
YÖKSİS Kayıtları
Effect of serotonin 7 receptors and transcript variants on RIN-5F pancreatic beta cell line
Toxicology in Vitro · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUHAMMED YAYLA →
Makale Bilgileri
DergiToxicology in Vitro
Yayın TarihiHaziran 2025
Cilt / Sayfa106
Scopus ID2-s2.0-86000539118
Özet
Purpose: The physiological effects of 5-HT7 receptors expressed in pancreatic beta cells have not yet been elucidated. We first aimed to investigate the effect of 5-HT7 receptor agonist (AS19) and antagonist (SB269970) application on insulin secretion in RIN-5F pancreatic beta cells. Subsequently, we aimed to investigate the effects of agonist and antagonist applications on cell damage induced by STZ. Materials/Methods: Cell damage was caused by giving 5 mM STZ solution to the cells for 12 h. The protective effects of 5-HT7 receptor agonist and antagonist on this subsequent damage were investigated. IGF-1, TNF-α, TGF-B1, NF-KB, Bax, Caspase 3, Caspase 9, 5-HT7, 5HT7x2, 5HT7x3 mRNA expression levels were compared between groups. Results: While agonist application stimulates insulin secretion, the effect of the antagonist varies.SB269970 reduced oxidative stress and downregulated TNF-a, TGF-B1 and NF-KB expression and also prevented apoptosis by decreasing Bax, caspase 3 and 9 levels against STZ-induced beta cell damage. Conclusions: The effect of 5-HT7 receptors on insulin secretion and their effects against STZ damage will be guiding for more detailed studies in the treatment of diabetes and related diseases in the future.
Yazarlar (6)
1
Muhammed Yayla
ORCID: 0000-0002-0659-3084
2
Erdem Toktay
ORCID: 0000-0002-0715-2707
3
Bengul Ozdemir Sarikaya
4
Ugur Ermis
5
Sakir Akgun
6
Irfan Cinar
ORCID: 0000-0002-9826-2556
Anahtar Kelimeler
5-HT7
AS19
Insulin
RIN-5F
SB269970
Kurumlar
Kafkas Üniversitesi
Kars Turkey
Kastamonu University
Kastamonu Turkey
Selçuk Tip Fakültesi
Konya Turkey