Scopus
YÖKSİS Eşleşti
Antioxidant effects of supplementation of 3,4-dihydroxyphenyl glycol on sperm parameters and oxidative markers following cryopreservation in canine semen
Reproduction in Domestic Animals · Temmuz 2021
YÖKSİS Kayıtları
Antioxidant effects of supplementation of 3,4\u2010dihydroxyphenyl glycol on sperm parameters and oxidative markers following cryopreservation in canine semen
Reproduction in Domestic Animals · 2021 SCI-Expanded
PROFESÖR MUSTAFA NUMAN BUCAK →
Makale Bilgileri
DergiReproduction in Domestic Animals
Yayın TarihiTemmuz 2021
Cilt / Sayfa56 · 1004-1014
Scopus ID2-s2.0-85105910606
Özet
Supplementing the extender with antioxidants with low molecular weight can enhance the quality of the post-thaw sperm during the freezing process. This study was aimed at determining the impacts of 3,4-dihydroxyphenyl glycol (DHPG) on the spermatozoa of the canine undergoing freeze-thawing process. In this study, 24 ejaculates were obtained from three mixed-breed dogs and were diluted in a Tris-based extender. The diluted semen was divided into aliquots for supplementation of 10, 30, 50 and 70 µg/ml of DHPG, control (without antioxidant) and control sham (DMSO). After being extended, the semen was equilibrated at a temperature of 4°C and then transferred to the straws and kept 4 cm above the liquid nitrogen for 20 min and was finally immersed in the liquid nitrogen. They were cryopreserved for seven days; then, sperm parameters including sperm motility evaluation, motility characteristics, viability, DNA and plasma membrane integrity, total antioxidant capacity (TAC), reduced glutathione content (GSH), antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT] and glutathione peroxidase [GPx]) activity malondialdehyde (MDA) levels were evaluated. This study showed that spermatozoa cryopreservation with 50, 30 and 70 µg/ml of DHPG concentrations had better progressive motility, Curvilinear Velocity, Linearity, viability, intact plasma membrane and the levels of TAC, GPx and GSH were higher than the control group. The 50, 30 and 70 µg/ml of DHPG concentrations led to the significant decrease of DNA damage compared to the control group. Total motility, average path velocity, straight-line velocity and CAT activity were significantly improved in 30 and 50 µg/ml of DHPG concentrations, compared to the control group. Also, the 50 and 30 µg/ml of DHPG concentrations, decreased MDA levels compared to the other groups, significantly. In conclusion, our study showed that the addition 50 µg/ml of DHPG to the canine semen extender improved the semen characteristics and oxidative markers in the cryopreservation process.
Yazarlar (4)
1
Nastaran Shakouri
2
Ali Soleimanzadeh
ORCID: 0000-0002-1591-2198
3
Alaleh Rakhshanpour
4
Mustafa Numan Bucak
Anahtar Kelimeler
3,4-dihydroxyphenyl glycol
canine
cryopreservation
oxidative markers
sperm parameters
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Urmia University Faculty of Veterinary Medicine
Urmia Iran
Metrikler
13
Atıf
4
Yazar
5
Anahtar Kelime