Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Separation and identification of different therapeutic pharmaceutical drugs on humic acid-based HPLC column: Method optimization using central composite design
Journal of Separation Science · Temmuz 2023
YÖKSİS Kayıtları
Separation and identification of different therapeutic pharmaceutical drugs on humic acid‐based HPLC column: Method optimization using central composite design
Wiley · 2023 SCI-Expanded
PROFESÖR HAMİDE FİLİZ AYYILDIZ →
Separation and identification of different therapeutic pharmaceutical drugs on humic acid‐based HPLC column: Method optimization using central composite design
Wiley · 2023 SCI-Expanded
PROFESÖR HÜSEYİN KARA →
Makale Bilgileri
DergiJournal of Separation Science
Yayın TarihiTemmuz 2023
Cilt / Sayfa46
Scopus ID2-s2.0-85161351625
Erişim🔓 Açık Erişim
Özet
Multimode chromatographic separations are highly desirable in pharmaceutical and environmental sciences. Current study deals with the application of newly developed mixed-mode end capped-immobilized humic acid onto an aminopropyl silica based chromatographic column for separation and identification of six drugs belonging to different therapeutic groups for its applicability in pharmaceutical industries. For this, central composite design was used to evaluate the separation and resolution by optimization of three most effective parameters (acetonitrile%, flow rate, and pH of mobile phase). Second-order quadratic model was used to evaluate their effect on resolution of peaks; the probability value (<0.05) obtained from analysis of variance suggested the best applicability of the model. Desirability function was applied to calculate optimum conditions (44.8% acetonitrile, 1.75 mL/min of flow rate, and 7.5 pH) required to achieve maximum separation with good resolution within 11 min. The method was validated for linearity, precision accuracy, selectivity, and sensitivity. The results revealed a highly precise (coefficient of variance > 1%), linear (R2 = 0.99), and highly selective method. Moreover, the limit of detection/quantification values revealed acceptable sensitivity of the method. The developed column was compared for its efficiency with a commercially available column and found to be highly applicable for industrial applications.
Yazarlar (4)
1
Hamide Filiz Ayyildiz
2
Fakhar N. Memon
ORCID: 0000-0002-8700-5597
3
Irfan Ali Mangi
4
Huseyin Kara
Anahtar Kelimeler
aminopropyl silica
central composite design
chromatography
humic acid
therapeutic drugs
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
University of Karachi
Karachi Pakistan
Metrikler
1
Atıf
4
Yazar
5
Anahtar Kelime