Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Tetraselmis chuii Edible Microalga as a New Source of Neuroprotective Compounds Obtained Using Fast Biosolvent Extraction
International Journal of Molecular Sciences · Nisan 2024
YÖKSİS Kayıtları
Tetraselmis chuii Edible Microalga as a New Source of Neuroprotective Compounds Obtained Using Fast Biosolvent Extraction
International Journal of Molecular Sciences · 2024 SCI
PROFESÖR HÜSEYİN KARA →
Tetraselmis chuii Edible Microalga as a New Source of Neuroprotective Compounds Obtained Using Fast Biosolvent Extraction
International Journal of Molecular Sciences · 2024 SCI
ÖĞRETİM GÖREVLİSİ MELİS ÇOKDİNLEYEN →
Makale Bilgileri
DergiInternational Journal of Molecular Sciences
Yayın TarihiNisan 2024
Cilt / Sayfa25
Scopus ID2-s2.0-85190362185
Erişim🔓 Açık Erişim
Özet
Tetraselmis chuii is an EFSA-approved novel food and dietary supplement with increasing use in nutraceutical production worldwide. This study investigated the neuroprotective potential of bioactive compounds extracted from T. chuii using green biobased solvents (ethyl acetate, AcOEt, and cyclopentyl methyl ether, CPME) under pressurized liquid extraction (PLE) conditions and supercritical fluid extraction (SFE). Response surface optimization was used to study the effect of temperature and solvent composition on the neuroprotective properties of the PLE extracts, including anticholinergic activity, reactive oxygen/nitrogen species (ROS/RNS) scavenging capacity, and anti-inflammatory activity. Optimized extraction conditions of 40 °C and 34.9% AcOEt in CPME resulted in extracts with high anticholinergic and ROS/RNS scavenging capacity, while operation at 180 °C and 54.1% AcOEt in CPME yielded extracts with potent anti-inflammatory properties using only 20 min. Chemical characterization revealed the presence of carotenoids (neoxanthin, violaxanthin, zeaxanthin, α- and β-carotene) known for their anti-cholinesterase, antioxidant, and anti-inflammatory potential. The extracts also exhibited high levels of omega-3 polyunsaturated fatty acids (PUFAs) with a favorable ω-3/ω-6 ratio (>7), contributing to their neuroprotective and anti-inflammatory effects. Furthermore, the extracts were found to be safe to use, as cytotoxicity assays showed no observed toxicity in HK-2 and THP-1 cell lines at or below a concentration of 40 μg mL−1. These results highlight the neuroprotective potential of Tetraselmis chuii extracts, making them valuable in the field of nutraceutical production and emphasize the interest of studying new green solvents as alternatives to conventional toxic solvents.
Yazarlar (8)
1
Melis Cokdinleyen
2
Gerardo Alvarez-Rivera
ORCID: 0000-0003-1657-8141
3
Jose Luis González Tejera
4
José A. Mendiola
ORCID: 0000-0001-6709-349X
5
Alberto Valdés
ORCID: 0000-0002-7901-5816
6
Huseyin Kara
7
Elena Ibáñez
ORCID: 0000-0003-2127-8303
8
Alejandro Cifuentes
ORCID: 0000-0002-7464-0217
Anahtar Kelimeler
carotenoids
green extraction
neuroprotective effect
polyunsaturated fatty acids
pressurized liquid extraction
QTOF
Tetraselmis chuii
Kurumlar
CSIC-UAM - Instituto de Investigacion en Ciencias de la Alimentacion (CIAL)
Madrid Spain
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
2
Atıf
8
Yazar
7
Anahtar Kelime