Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Comparison of microbiota and volatile organic compounds in milk from different sheep breeds
Journal of Dairy Science · Aralık 2021
YÖKSİS Kayıtları
Comparison of microbiota and volatile organic compounds in milk from different sheep breeds
Journal of Dairy Science · 2021 SCI-Expanded
DOÇENT YUSUF BİÇER →
Comparison of microbiota and volatile organic compounds in milk from different sheep breeds
Journal of Dairy Science · 2021 SCI-Expanded
DOÇENT ARİFE EZGİ TELLİ →
Comparison of microbiota and volatile organic compounds in milk from different sheep breeds
Journal of Dairy Science · 2021 SCI-Expanded
PROFESÖR GÜRKAN UÇAR →
Comparison of microbiota and volatile organic compounds in milk from different sheep breeds
Journal of Dairy Science · 2021 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ GONCA SÖNMEZ →
Makale Bilgileri
DergiJournal of Dairy Science
Yayın TarihiAralık 2021
Cilt / Sayfa104 · 12303-12311
Scopus ID2-s2.0-85115933081
Erişim🔓 Açık Erişim
Özet
In this study, we compared the microbiota and volatile organic compounds (VOC) present in the milk obtained from 3 different sheep breeds, namely Merino, Lacaune, and Assaf. Udder milk was collected from 21 animals, 7 from each breed. Bacterial microflora was determined metagenomically by extracting the DNA from the milk and analyzing the V3-V4 region of the 16S rRNA gene. Headspace solid-phase microextraction gas chromatography-mass spectrometry method was used to analyze VOC. The metagenomic analysis revealed (for Merino, Lacaune, and Assaf milk, respectively) Firmicutes (66.32, 69.36, and 57.08%), Actinobacteria (19.09, 7.67, and 19.40%), Proteobacteria (13.76, 21.06, and 22.19%), and Bacteroidetes (0.84, 1.91, and 1.33%) phyla in the milk samples. Lactobacillus was highly abundant in the milk of 3 breeds (29.64, 43.50, and 18.70%). The genera constituting more than 2% of all bacteria in all groups were Jeotgalicoccus (7.19, 5.34, and 10.77%), Enterococcus (5.18, 9.78, and 3.64%), and Corynebacterium (4.08, 3.00, and 13.44%). A total of 32 different VOC were identified by headspace solid-phase microextration analysis with 9, 30, and 24 different compounds from Merino, Lacaune, and Assaf breeds, respectively. Although ketone was the most abundant compound in Merino milk (71.84%), hydrocarbons were the most detected in Lacaune and Assaf milk (37.18% and 55.42%, respectively). A positive correlation was found between acetone, which was detected at the highest level in all groups, with Salinicoccus, Alloiococcus, Psychrobacter, and Dietzia. In addition, a negative correlation was found between the Lactobacillus genus, detected at the highest level in all groups, with methyl cyclopentane, 3-methylheptane, octane, decane, 3,3-dimethyloctane, and dodecane. Thus, differences were observed in the bacterial microflora and VOC in the sheep milk from different breeds under different feeding and breeding conditions.
Yazarlar (5)
1
Yusuf Biçer
ORCID: 0000-0001-7549-8323
2
A. Ezgi Telli
3
Gonca Sönmez
4
Nihat Telli
5
Gürkan Uçar
Anahtar Kelimeler
16S rRNA
headspace solid-phase microextraction gas chromatography-mass spectrometry
metagenomic analysis
sheep milk
volatile organic compound
Kurumlar
Konya Technical University
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
11
Atıf
5
Yazar
5
Anahtar Kelime