Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Effects of gamma ray sources and irradiation doses on the haploidization frequency of citron watermelon (Citrullus lanatus var. citroides)
Plant Cell, Tissue and Organ Culture · Mart 2025
YÖKSİS Kayıtları
Effects of gamma ray sources and irradiation doses on the haploidization frequency of citron watermelon (Citrullus lanatus var. citroides)
Plant Cell, Tissue and Organ Culture (PCTOC) · 2025 SCI-Expanded
ARAŞTIRMA GÖREVLİSİ ÜNAL KAL →
Effects of gamma ray sources and irradiation doses on the haploidization frequency of citron watermelon (Citrullus lanatus var. citroides)
Plant Cell, Tissue and Organ Culture (PCTOC) · 2025 SCI-Expanded
DOÇENT MUSA SEYMEN →
Makale Bilgileri
DergiPlant Cell, Tissue and Organ Culture
Yayın TarihiMart 2025
Cilt / Sayfa160
Scopus ID2-s2.0-85218418361
Erişim🔓 Açık Erişim
Özet
Drought is widely regarded as the most significant abiotic stressor affecting modern agricultural production. Sweet watermelon (Citrullus lanatus var. lanatus), one of the world’s most cultivated vegetable species, lacks drought resistance and must be grown in dry and semi-arid regions by grafting on tolerant rootstocks. Citron watermelon (Citrullus lanatus var. citroides) is well known for its high drought tolerance and has excellent potential as a watermelon rootstock. There is a high demand for novel citron watermelon cultivars with rootstock potential. Doubled haploid (DH) technology enables the breeding of genetically uniform and fully homozygous plants in a single generation. However, no research has been conducted on the haploidization of citron watermelon using the irradiated pollen technique. The current study aimed to determine the efficiency of gamma-ray sources (Cobalt: Co60 and Cesium: Cs137), irradiation doses (200, 250, and 300 Gy of Co60; 100, 150, 200, and 250 Gy of Cs137), and genotypes (11 citron watermelon lines) on the production of pure citron lines via the irradiated pollen technique (parthenogenesis). The embryos were grown on MS medium supplemented with 0.4 mg/L indole-3-butyric acid. Stomatal observations and flow cytometry were used to conduct ploidy studies on 3–4 weeks old parthenogenic plantlets. The Co60 treatments yielded eight haploid and two mixoploid plants, while the Cs137 treatments yielded 14 haploid and one mixoploid. These findings demonstrated that gamma rays from both radiation sources were effective in parthenogenic embryo induction, and Cs137 was discovered to be a reliable alternative to conventional Co60.
Yazarlar (9)
1
Ertan Sait Kurtar
2
Deniz Metin
3
Zeliha Atakul
4
Ünal Kal
5
Musa Seymen
6
Enes Takcı
ORCID: 0009-0002-1189-0021
7
Hatice Nur Yılmaz
ORCID: 0009-0008-1156-4094
8
Ali Ramazan Alan
9
Fevziye Çelebi Toprak
ORCID: 0000-0002-0197-9949
Anahtar Kelimeler
Citron watermelon
Haploidization
Irradiation sources
Rootstock
Kurumlar
Pamukkale Üniversitesi
Denizli Turkey
Sakarya University of Applied Sciences
Serdivan Turkey
Selçuk Üniversitesi
Selçuklu Turkey