Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
The clinical efficacy of cGMP-specific sildenafil on mitochondrial biogenesis induction and renal damage in cats with acute on chronic kidney disease
BMC Veterinary Research · Aralık 2024
YÖKSİS Kayıtları
The clinical efficacy of cGMP-specific sildenafil on mitochondrial biogenesis induction and renal damage in cats with acute on chronic kidney disease
BMC Veterinary Research · 2024 SCI-Expanded
ARAŞTIRMA GÖREVLİSİ SÜLEYMAN SERHAT İYİGÜN →
The clinical efficacy of cGMP-specific sildenafil on mitochondrial biogenesis induction and renal damage in cats with acute on chronic kidney disease
BMC Veterinary Research · 2024 SCI-Expanded
DOÇENT AMİR NASERİ →
The clinical efficacy of cGMP-specific sildenafil on mitochondrial biogenesis induction and renal damage in cats with acute on chronic kidney disease
BMC Veterinary Research · 2024 SSCI
PROFESÖR MEHMET MADEN →
The clinical efficacy of cGMP-specific sildenafil on mitochondrial biogenesis induction and renal damage in cats with acute on chronic kidney disease
BMC Veterinary Research · 2024 SCI-Expanded
DOÇENT MERVE İDER →
Makale Bilgileri
DergiBMC Veterinary Research
Yayın TarihiAralık 2024
Cilt / Sayfa20
Scopus ID2-s2.0-85208161722
Erişim🔓 Açık Erişim
Özet
Background: Mitochondrial biogenesis (MB) induction has recently emerged as potential therapeutic approaches in kidney pathology and the mitochondria-targeted therapies should be investigated to improve treatment of animals with kidney diseases. This study aimed to investigate the effects of MB induction with sildenafil citrate on the cGMP/NO pathway, glomerular filtration, and reduction of kidney damage and fibrosis (TGF-β/SMAD pathway) in cats with acute on chronic kidney disease (ACKD). Thirty-three cats were divided into the non-azotemic (healthy) group (n:8) and the ACKD group (n:25), comprising different breeds, sexes, and ages. Sildenafil citrate was administered to the non-azotemic and ACKD groups (2.5 mg/kg, PO, q12 hours) for 30 days. Serum and urine NO, MDA, NGAL, KIM-1, TGF-β1, IL-18, FGF 23, PGC-1α and cGMP concentrations were measured. Results: Serum cGMP concentrations increased (P < 0.05) in the non-azotemic group during the 2nd (median 475.99 pmol/mL) and 3rd (median 405.01 pmol/mL) weeks of the study, whereas serum cGMP concentrations decreased in the ACKD group during the 4th(median 188.52 pmol/mL) week compared to the non-azotemic group (P < 0.05). No difference was observed in serum biomarker concentrations except NO, which increased in the 4th week (P < 0.05). The urinary concentrations of NO, MDA, PGC-1α, TGF-β1, NGAL, KIM-1, IL-18, and FGF 23 in the ACKD group were found to be higher compared to those in the non-azotemic group from the 1st to the 4th week (P < 0.05). In the ACKD group, the urine PGC-1α concentration in the 2nd (median 6.10 ng/mL) week was lower compared to that in the 0 and 1st (median 7.65 and 7.21 ng/mL, respectively) week, and the NO concentration in the 3rd (median 28.94 µmol/mL) week was lower than that in the 0th (median 37.43 µmol/mL) week (P < 0.05). Conclusions: While sildenafil citrate has been determined to induce a low level of MB and to have a beneficial effect on glomerular filtration, it is observed to be ineffective in mitigating renal damage and fibrosis via the TGF-β/SMAD pathway in cats with ACKD.
Yazarlar (12)
1
Mehmet Maden
2
Merve Ider
ORCID: 0000-0003-2928-5452
3
Mehmet Erman Or
ORCID: 0000-0002-8764-1956
4
Banu Dokuzeylül
ORCID: 0000-0003-3086-4726
5
Erdem Gülersoy
ORCID: 0000-0001-8511-0150
6
Merve Cansu Kilickaya
7
Bengü Bilgiç
ORCID: 0000-0002-6952-2937
8
Murat Kaan Durgut
9
Semih İzmirli
ORCID: 0000-0002-1781-0494
10
Suleyman Serhat Iyigun
11
Deniz Zeynep Telci
ORCID: 0000-0001-6825-2093
12
Amir Naseri
Anahtar Kelimeler
Azotemia
Cat
Mitochondrial biogenesis
Sildenafil citrate
Urinary biomarker
Kurumlar
Harran Üniversitesi
Sanliurfa Turkey
Istanbul University-Cerrahpasa
Istanbul Turkey
Selçuk Üniversitesi
Selçuklu Turkey