Scopus
YÖKSİS ISSN Eşleşti
SJR Q4
Diagnosis of bovine tuberculosis by PPD-ELISA and sonication-ELISA
Israel Journal of Veterinary Medicine · Aralık 2013
YÖKSİS Kayıtları — ISSN Eşleşmesi
Three Dimensional Reconstruction of Stomach and Intestines in New Zealand White Rabbits from Computerized Tomography Images
2011 ISSN: 0334-9152 SCI-Expanded
Prof. Dr. MUSTAFA ORHUN DAYAN →
Haemosporidian parasites in blood smears of sparrowhawks Accipiter nisus Falconiformes Accipitridae in northeastern Turkey
2016 ISSN: 0334-9152 SCI-Expanded
Prof. Dr. ERTAN ORUÇ →
Clinical Experience of Interlocking Nail Stabilization of Long Bone Fractures in Dogs – A Retrospective Study of 26 Cases
2017 ISSN: 0334-9152 SCI-Expanded
Prof. Dr. MUSTAFA ARICAN →
Clinical Experience of Interlocking Nail Stabilization of Long Bone Fractures in Dogs - A Retrospective Study of 26 Cases
2017 ISSN: 0334-9152 SCI-Expanded Q4
Doç. Dr. KURTULUŞ PARLAK →
Comparison of Instilled Lidocaine and Procaine Effects on Pain Relief in Dogs Undergoing Elective Ovariohysterectomy
2019 ISSN: 0334-9152 SCI-Expanded
Prof. Dr. HASAN GÜZELBEKTEŞ →
Diagnosis of Bovine Tuberculosis by PPD-ELISA and Sonication-ELISA
2014 ISSN: 0334-9152 SCI-Expanded
Prof. Dr. OSMAN ERGANİŞ →
Subclinical Myocardial Injury in Clinically Healthy Obese Dogs
2020 ISSN: 0334-9152 SCI-Expanded Q4
Prof. Dr. MAHMUT OK →
Efficacy of oral transmucosal administrations of phenylbutazone for postoperative analgesia in dogs undergoing ovariohysterectomy
2021 ISSN: 0334-9152 SCI-Expanded Q4
Prof. Dr. HASAN GÜZELBEKTEŞ →
Immunohistochemical Detection of Tissue Localization of Acute Phase Proteins in Cattle Pneumonic Pasteurellosis
2023 ISSN: 0334-9152 CABI, EBSCO, Scobus
Prof. Dr. MUSTAFA ORTATATLI →
Makale Bilgileri
ISSN03349152
Yayın TarihiAralık 2013
Cilt / Sayfa68 · 180-184
Scopus ID2-s2.0-84890845777
Özet
Mycobacterium bovis (M. bovis) infection in cattle remains a major zoonotic and economic problem in many countries. In addition, M. bovis is an important pathogen of both man and animals. The standard diagnostic test for this disease is the intradermal tuberculin test, one of the oldest immunological tests still in widespread use. However, this test can lack both sensitivity and specificity. Serological tests such as ELISA (Enzyme Linked Immunosorbent Assay) have been suggested as an alternative method for the diagnosis of tuberculosis. In the present study, two serum ELISAs, performed for the detection of M. ¿ww-specific antibodies, were evaluated against the culture of tissue samples from 135 slaughtered dairy cows, which were intradermal tuberculin-test-positive. ELISA plates were coated with Bovine Purified Protein Derivative (PPD B) and M. bovis-sonication antigen. Samples were taken from the bronchial (BLN), mesenteric (MLN) and prescapular (PLN) lymph nodes and tubercules (TB) of slaughtered cattle. Tissue samples were cultured in Lowenstein-Jensen medium. Blood samples, which were collected from the tuberculin-test-positive animals before slaughter, were tested by PPD B-ELISA (PPD-ELISA) and M. bovis sonication-ELISA (sonication-ELISA). Mycobacterium spp. was isolated from 77 out of 135 cattle. Out of 135 cattle, 62 (45.9%) and 43 (31.8%) were determined to be positive for PPD-ELISA and sonication-ELISA, respectively. Using culture-positive animals as a gold standard, the sensitivity and specificity of these two methods were detected as 48.2%-55.2% and 31.2%-44.5%, respectively.
Yazarlar (2)
1
Zafer Sayın
2
O. Erganiş
Anahtar Kelimeler
Bovine tuberculosis
Mycobacterium bovis
PPD-ELISA
Sonication-ELISA
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Israel Journal of Veterinary Medicine
Q4
SJR Skoru0,176
H-Index16
YayıncıIsrael Veterinary Medical Association
ÜlkeIsrael
Animal Science and Zoology (Q4)
Veterinary (miscellaneous) (Q4)
Metrikler
7
Atıf
2
Yazar
4
Anahtar Kelime