Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q4
Investigations of pathological immunohistochemical and immunocytochemical findings in natural infection with Mycoplasma gallisepticum in laying hens
Acta Scientiae Veterinariae · Ocak 2019
YÖKSİS Kayıtları
Investigations of Pathological Immunohistochemical and Immunocytochemical Findings in Natural Infection with Mycoplasma gallisepticum in Laying Hens
Acta Scientiae Veterinariae · 2019 SCI
Prof. Dr. ÖZGÜR ÖZDEMİR →
Investigations of Pathological Immunohistochemical and Immunocytochemical Findings in Natural Infection with Mycoplasma gallisepticum in Laying Hen
Acta Scientiae Veterinariae · 2019 SCI-Expanded
Prof. Dr. ZAFER SAYIN →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Bovine Leukemia Virus Antibodies in Dairy Cattle Farms and Milk Cooling Tanks
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Effect of CorynebacteriumcutisLysate on Serum Oxidative Stressand Plasma Prostaglandin F2 Metabolite Levels
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Hepatoprotective Effects of Ginseng in Rats Fed Cholesterol Rich Diet
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ERCAN KESKİN →
Effect of Anti TNF on the Development of Offspringand Pregnancy Loss During Pregnancy in Rats
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. AYŞE ER →
Investigation of the Effect of Tarantula cubensis Extract on Acute Phase Response
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. AYŞE ER →
Detection of Antibodies against Equine Herpes Virus 1and Equine Herpes Virus 4 in Horses in Southeast Anatolia by Indirect Elisa
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Hepatoprotective Effects of Ginseng in Rats Fed Cholesterol Rich Diet
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. DENİZ ULUIŞIK →
Investigation of the Effect of Tarantula cubensis Extract on Acute Phase Response
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Effect of Anti TNF-a on the Development of Offspringand Pregnancy Loss During Pregnancy in Rats
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Amiodarone May Prevent the Tilmicosin caused Lethal Toxicity
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BÜNYAMİN TRAŞ →
Effect of Hoof Trimming on Milk Yield in Dairy Cows with Foot Disease
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. TAMER ÇAĞLAYAN →
Bovine coronavirus (BoCV) infection in calves with diarrhoea and their dams
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ORHAN YAPICI →
The Effects of Coenzyme Q10 on Inflammation Markers in Streptozotocin-Induced Diabetic Rats
2017 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ERCAN KESKİN →
The Effects of Coenzyme Q10 on Inflammation Markersin Streptozotocin-Induced Diabetic Rats
2017 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. DENİZ ULUIŞIK →
Comparison of the Effectiveness of Halofuginone Lactate and Paromomycin in the Treatment of Calves Naturally Infected with Cryptosporidium parvum
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. NERMİN IŞIK USLU →
Safety of antitheilerial drug buparvaquone in rams
2018 ISSN: 1678-0345 SCI-Expanded Q4
Prof. Dr. NERMİN IŞIK USLU →
Pentoxifylline May Restore Kanamycin-Induced Renal Damage in Rats
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ÖZGÜR ÖZDEMİR →
Comparison of the Effectiveness of Halofuginone Lactate and Paromomycin in the Treatment of Calves Naturally Infected with Cryptosporidium parvum
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ÖZLEM DERİNBAY EKİCİ →
Comparison of Thermographic Imaging and Other Diagnostic Techniques in Diagnosis of Cattle with Laminitis
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. FATİH HATİPOĞLU →
Bovine Coronavirus (BoCV) Infection in Calves with Diarrhoea and Their Dams
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Makale Bilgileri
ISSN16780345
Yayın TarihiOcak 2019
Cilt / Sayfa47
Scopus ID2-s2.0-85087642827
Erişim🔓 Açık Erişim
Özet
Background: Mycoplasmosis is an infectious disease caused by Mycoplasma gallisepticum (MG), usually seen in the respiratory system of chickens, chick and turkeys, that causing great economic loss. The disease is characterized by respiratory system lesions such as sinusitis, tracheitis, airsacculitis, pneumonia and other symptoms such as loss of yield, arthritis, tenosynovitis. In this study, it was aimed to investigate diagnose of the disease by pathologic and molecular techniques in hens that naturally infected with MG as well as the usability of immunocytochemical (ICC) method in diagnose of the disease. Materials, Methods & Results: For this purpose, 98 hens were collected from 10 different coops that serologically positive. After necropsy, routine pathological procedures were performed to samples taken from nose, sinus, larynx, trachea, lung and air sacs. Scraping samples taken from lungs and tracheas were evaluated by ICC. Immunohistochemical (IHC) staining was performed to samples taken from nose, sinus, larynx, trachea, lung and air sacs. Indirect immunoperoxidase method was applied in the both IHC and ICC staining. Rabbit polyclonal anti MG antibody was used as primer antibody in the IHC and ICC staining. Additionally, culture and PCR techniques were applied to tracheas of all hens for MG. The GPO3 and MGSO genes were made for PCR analysis. In the tracheal examinations, 23 cases were positive for PCR, 17 cases ICC positive, 16 cases IHC positive and 10 culture samples found positive. All of culture positive cases were also positive for other three methods. When findings in all organs were evaluated, in 37 cases were detected positive by IHC (38%) and 23 cases were positive by ICC (23.5%). In the IHC positive cases, the first order was trachea in 16 cases followed by in 11 cases in sinus, in 8 cases in lung, in 6 cases air sac and 4 cases in nose, respectively. In 8 cases, IHC positivity was found in at least two organs. IHC positivity was detected in the nose, sinus and tracheal epithelia as well as in the macrophages within subepithelial lymphoid infiltration, vascular walls and endothelium. As the disease became chronic, it was found that the agents were seen more in the lymphoid tissue than the epithelium. In ICC staining positivity was found in 17 cases in the trachea and 11 cases in the lung. There were only 5 cases positive by ICC in both organs. Discussion: Clinical and pathological findings as well as serological, microbiological, molecular techniques and immunohistochemical methods are to be important methods in the diagnosis of the disease. While the culture results are shown as the gold standard in diagnosis of the disease, it is possible to obtain the results in the earliest 7-10 days in cultures and at least 20 days must be passed in order to say a cultural negative. In addition, in the field studies, it mentioned the use of vaccines, antibiotics and protective drugs affected the results of microbiology and serology; the importance of using techniques such as IHC and PCR for the diagnosis of the causative agents. The results of the present study indicate that the most important organ in the diagnosis of the disease is the trachea, and the most effective method is PCR followed by IHC and ICC methods. It was concluded that the results of ICC staining close to IHC staining, and ICC could be used for diagnostic purposes in positive reactions obtained from the tracheas or the other organs.
Yazarlar (4)
1
O. Ozdemir
2
Orhan Yavuz
3
H. Erer
4
Zafer Sayın
Anahtar Kelimeler
Fast diagnosis
Immunocytochemistry
Immunohistochemistry
Pathology
PCR
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Acta Scientiae Veterinariae
Q4
SJR Skoru0,155
H-Index20
YayıncıUniversidade Federal do Rio Grande do Sul
ÜlkeBrazil
Veterinary (miscellaneous) (Q4)
Metrikler
1
Atıf
4
Yazar
5
Anahtar Kelime