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Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19

Polish Journal of Veterinary Sciences · Ocak 2022

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YÖKSİS Kayıtları
Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19
Polish Journal of Veterinary Sciences · 2022 SCI-Expanded
PROFESÖR OSMAN ERGANİŞ →
Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19
Polish Journal of Veterinary Sciences · 2022 SCI-Expanded
DOÇENT ALİ USLU →
Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19
Polish Journal of Veterinary Sciences · 2022 SCI-Expanded
PROFESÖR ZAFER SAYIN →

Makale Bilgileri

DergiPolish Journal of Veterinary Sciences
Yayın TarihiOcak 2022
Cilt / Sayfa25 · 561-569
Özet Brucellosis is a worldwide zoonosis, that can still be classified as endemic despite its ancient origins which causes economic losses and public health problems. Although effectively controlled by vaccination in animals, there is currently no vaccine for use in humans. Outer Membrane Proteins (OMP) that play an active immunogenic and protective role in the Brucellae family. OMP19 is present in all Brucella species as a surface antigen and is a potent immunogen responsible for Brucellosis intracellular infection. For this reason, the study was aimed to be used safely as a potential recombinant vaccine candidate against all Brucella infections, especially in humans and pregnant animals. This study evaluated a Brucella lipoprotein antigen, i.e. 19 kilodalton (kDa) outer membrane protein (OMP19), which was amplified and cloned into the pETSUMO vector system. The immunogenic power of the purified recombinant OMP19 antigen against brucellosis was compared with that of OMP19 (Raybiotech Inc, USA) in a mouse model and the obtained rOMP19 antigen was found to be similar to the commercially available recombinant protein.

Yazarlar (8)

1
Ali Uslu
2
G. Sanioglu Golen
3
M. Agah Tekindal
4
Aslı Sakmanoğlu
5
Zafer Sayın
6
O. Denizli
7
A. Gok
8
O. Erganiş

Anahtar Kelimeler

Brucella melitensis recombinant protein rOMP19 antigen

Kurumlar

Aksaray Üniversitesi
Aksaray Turkey
İzmir Kâtip Çelebi Üniversitesi
Izmir Turkey
Selçuk Üniversitesi
Selçuklu Turkey