Scopus
YÖKSİS Eşleşti
Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages
Microscopy Research and Technique · Nisan 2016
YÖKSİS Kayıtları
Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages
Microscopy Research and Technique · 2016 SCI-Expanded
PROFESÖR İLHAMİ ÇELİK →
Makale Bilgileri
DergiMicroscopy Research and Technique
Yayın TarihiNisan 2016
Cilt / Sayfa79 · 251-257
Scopus ID2-s2.0-84962524923
Özet
The aim of the present study was to investigate immunohistochemical distribution of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the mouse placenta at different gestational stages. For this purpose a total of 18 Swiss albino female mice at 12-14 weeks of age were used. Females were sacrificed on days 3 (early), 10 (mid-), and 17 (late) of pregnancy and the implantation sites of the pregnant uterus were sampled. The sections were made transversely through the central region of the implantation site and stained with hematoxylin and eosin for histological examination. PCNA and Hsp70 was stained immunohistochemically. Since the definitive placenta was not still formed on day 3 of pregnancy, Hsp70 and PCNA positivity were evaluated in only luminal epithelium and decidual-stromal cells. On days 10 and 17 of pregnancy, Hsp70 and PCNA positivity were evaluated in labyrinth zone, junctional zone and decidual layer of placenta. Hsp70 expression was observed trophoblast cells and decidual cells and was relatively constant throughout the pregnancy. This protein was strongly labeled in the trophoblast cells; while decidual cells were displayed moderate staining. In early pregnant mouse uteri, PCNA was mainly localized in decidual-stromal cells. The trophoblast cells and decidual cells displayed highly proliferative activity at the midgestational period. However there was a significant decrease in the percentage of PCNA positive cells in late gestation.
Yazarlar (5)
1
Tuǧba Özaydin
2
Emrah Sur
3
Yasemin Öznurlu
4
Ilhami Çelik
5
Deniz Uluíşik
Anahtar Kelimeler
Decidualization
Hsp70
Mouse
PCNA
Placentation
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
9
Atıf
5
Yazar
5
Anahtar Kelime