Scopus
Formulation and testing of cutting-edge food supplements tailored for glucose and oxidation controlling, converting artichoke by-products in inulin-rich antioxidant phytocomplex loaded into zein liposomes
Food Bioscience · Aralık 2024
Makale Bilgileri
DergiFood Bioscience
Yayın TarihiAralık 2024
Cilt / Sayfa62
Scopus ID2-s2.0-85209091960
Özet
Agri-food by-products cause significant environmental and annual market losses. This study addresses the challenge of sustainable use of these by-products by developing an innovative food supplement from artichoke residues, rich in active molecules such as inulin and polyphenols. Its key components were identified and quantified using high-performance liquid chromatography with diode-array detection (HPLC-DAD), loaded into zein liposomes for oral delivery, and evaluated in vitro for biological effects. Zein liposomes were characterized by a small size (≈132 nm), homogeneously dispersion (polydispersity index ≈ 0.19), a negative charge (around −44 mV), and an efficient incorporation of artichoke extract (>70%). Freeze-drying improved the stability of vesicle dispersions and small-Angle X-ray Scattering analysis confirmed that zein stabilized the vesicles under acidic conditions. Zein liposomes controlled the release of loaded molecules in gastrointestinal-simulating pH (at pH 1.20 ⁓ 21% of the chlorogenic acid equivalents were released) and inhibited α-amylase (≈∼0.90 μg of acarbose equivalents/mL) and α-glucosidase (∼0.97 μg of acarbose equivalents/mL) in vitro. Formulations were biocompatible (cell viability ≈ 90%) with Caco-2 cells and protected them from oxidative stress. Overall, the results suggest that zein liposomes loaded with artichoke by-product extract are well-suited as a food supplement or nutraceutical to boost nutritional value.
Yazarlar (13)
1
Ines Castangia
ORCID: 0000-0002-3100-8107
2
Francesco Corrias
ORCID: 0000-0002-1324-7681
3
Francisco Javier Leyva-Jiménez
4
Matteo Aroffu
5
Federica Fulgheri
ORCID: 0000-0002-7858-1605
6
Matteo Perra
ORCID: 0000-0002-9906-990X
7
Alessandro Atzei
ORCID: 0000-0002-5638-9591
8
Alessandra del Giudice
9
Gokhan Zengin
ORCID: 0000-0001-6548-7823
10
Gunes Ak
11
Alberto Angioni
ORCID: 0000-0001-7370-0903
12
Maria Manconi
13
Maria Letizia Manca
ORCID: 0000-0002-3787-257X
Anahtar Kelimeler
Caco-2 cells
Extract
Oral delivery
Phospholipid vesicles
Ready-made meals
Zero-waste
Kurumlar
Sapienza Università di Roma
Rome Italy
Selçuk Üniversitesi
Selçuklu Turkey
Universidad de Castilla-La Mancha
Ciudad Real Spain
Universidad de Granada
Granada Spain
Università degli Studi di Cagliari
Cagliari Italy
Metrikler
1
Atıf
13
Yazar
6
Anahtar Kelime