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Preparation of two new chiral metal-organic frameworks for lipase immobilization and their use as biocatalysis in the enantioselective hydrolysis of racemic naproxen methyl ester

International Journal of Biological Macromolecules · Aralık 2024

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YÖKSİS Kayıtları
Preparation of two new chiral metal-organic frameworks for lipase immobilization and their use as biocatalysis in the enantioselective hydrolysis of racemic naproxen methyl ester
International Journal of Biological Macromolecules · 2024 SCI-Expanded
PROFESÖR ELİF ÖZYILMAZ →
Preparation of two new chiral metal-organic frameworks for lipase immobilization and their use as biocatalysis in the enantioselective hydrolysis of racemic naproxen methyl ester
International Journal of Biological Macromolecules · 2024 SCI-Expanded
PROFESÖR MUSTAFA YILMAZ →

Makale Bilgileri

DergiInternational Journal of Biological Macromolecules
Yayın TarihiAralık 2024
Cilt / Sayfa282
Özet Considering the selective pharmacological activity of chiral drugs, it is important to develop new chiral materials to synthesize them. In this work, two new chiral MOFs (UiO-66@Np and UiO-66@Ib) were prepared by the covalent attachment of the chiral compounds (S-naproxen and S-ibuprofen) to the amine-functionalized Zr-MOF (UiO-66-NH2). Then, Candida rugosa lipase (CRL) was immobilized on these chiral MOFs to fabricate two new biocomposites (UiO-66@Np@CRL and UiO-66@Ib@CRL) as effective biocatalysts, which enable significant enhancement in the catalytic activity and enantioselectivity of lipase. The FTIR, SEM, EDX, TGA, and PXRD analyses were carried out to confirm the formation of the biocomposites. The catalytic performances of the biocomposites (UiO-66@Np@CRL and UiO-66@Ib@CRL) were evaluated in the typical hydrolysis of p-nitro-phenyl palmitate (p-NPP) and the catalytic enantioselective hydrolysis of (R,S)-naproxen methyl ester. Under optimal conditions, UiO-66@Np@CRL showed a higher enantiomeric excess of the substrate (ees) value and a 98 % and 50 % conversion rate, respectively, than that of UiO-66@Ib@CRL. Besides, an excellent enantioselectivity (E) value of 458 was obtained in the presence of the biocomposite (UiO-66@Np@CRL). In addition, it was observed that the catalytic activity, conversion rate and ees value of this composite (UiO-66@Np@CRL) remained almost unchanged in reuse after 6 months. The results showed that in enantioselective reactions, the highest conversion and enantioselectivity could be achieved when the chiral compound bound to the MOF and the model compound used are the same.

Yazarlar (5)

1
Sebahat Ascioglu
2
Elif Ozyilmaz
3
Ayse Yildirim
4
Serkan Sayin
5
Mustafa Yilmaz

Anahtar Kelimeler

Chiral-MOF Enantioselectivity Lipase immobilization S-naproxen

Kurumlar

Giresun Üniversitesi
Giresun Turkey
Selçuk Üniversitesi
Selçuklu Turkey

Metrikler

10
Atıf
5
Yazar
4
Anahtar Kelime

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