Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q4
The Effect of Adding L-Ergothioneine in Culture Medium on Blastocyst Development Rates
Acta Scientiae Veterinariae · Ocak 2022
YÖKSİS Kayıtları
The Effect of Adding L-Ergothioneine in Culture Medium on Blastocyst Development Rates
Acta Scientiae Veterinariae · 2022 SCI-Expanded
Doç. Dr. SAKİNE ÜLKÜM ÇİZMECİ →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Bovine Leukemia Virus Antibodies in Dairy Cattle Farms and Milk Cooling Tanks
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Effect of CorynebacteriumcutisLysate on Serum Oxidative Stressand Plasma Prostaglandin F2 Metabolite Levels
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Hepatoprotective Effects of Ginseng in Rats Fed Cholesterol Rich Diet
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ERCAN KESKİN →
Effect of Anti TNF on the Development of Offspringand Pregnancy Loss During Pregnancy in Rats
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. AYŞE ER →
Investigation of the Effect of Tarantula cubensis Extract on Acute Phase Response
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. AYŞE ER →
Detection of Antibodies against Equine Herpes Virus 1and Equine Herpes Virus 4 in Horses in Southeast Anatolia by Indirect Elisa
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Hepatoprotective Effects of Ginseng in Rats Fed Cholesterol Rich Diet
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. DENİZ ULUIŞIK →
Investigation of the Effect of Tarantula cubensis Extract on Acute Phase Response
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Effect of Anti TNF-a on the Development of Offspringand Pregnancy Loss During Pregnancy in Rats
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Amiodarone May Prevent the Tilmicosin caused Lethal Toxicity
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BÜNYAMİN TRAŞ →
Effect of Hoof Trimming on Milk Yield in Dairy Cows with Foot Disease
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. TAMER ÇAĞLAYAN →
Bovine coronavirus (BoCV) infection in calves with diarrhoea and their dams
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ORHAN YAPICI →
The Effects of Coenzyme Q10 on Inflammation Markers in Streptozotocin-Induced Diabetic Rats
2017 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ERCAN KESKİN →
The Effects of Coenzyme Q10 on Inflammation Markersin Streptozotocin-Induced Diabetic Rats
2017 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. DENİZ ULUIŞIK →
Comparison of the Effectiveness of Halofuginone Lactate and Paromomycin in the Treatment of Calves Naturally Infected with Cryptosporidium parvum
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. NERMİN IŞIK USLU →
Safety of antitheilerial drug buparvaquone in rams
2018 ISSN: 1678-0345 SCI-Expanded Q4
Prof. Dr. NERMİN IŞIK USLU →
Pentoxifylline May Restore Kanamycin-Induced Renal Damage in Rats
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ÖZGÜR ÖZDEMİR →
Comparison of the Effectiveness of Halofuginone Lactate and Paromomycin in the Treatment of Calves Naturally Infected with Cryptosporidium parvum
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ÖZLEM DERİNBAY EKİCİ →
Comparison of Thermographic Imaging and Other Diagnostic Techniques in Diagnosis of Cattle with Laminitis
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. FATİH HATİPOĞLU →
Bovine Coronavirus (BoCV) Infection in Calves with Diarrhoea and Their Dams
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Makale Bilgileri
ISSN16780345
Yayın TarihiOcak 2022
Cilt / Sayfa50
Scopus ID2-s2.0-85213879073
Erişim🔓 Açık Erişim
Özet
Background: Oocytes and embryos produce energy through mitochondrial oxidative phosphorylation by using oxygen. The membrane structure of the embryo is mostly composed of unsaturated fatty acids, for this reason DNA fragmentation, apoptosis, and abnormal gene expression are shaped as a result of the lipid peroxidation during culture. Oxidative stress (OS) is one of the most important problems affecting the in vitro embryo development. Antioxidant supplementation to the culture medium has been an alternative way to reduce cell damage caused by oxidative stress in in vitro embryo production systems. In this study, it was aimed to determine the effect of L-ergothioneine on blastocyst development when added to the culture medium. Materials, Methods & Results: The material of the study consisted of oocytes aspirated from the ovaries of Holstein cows which were collected from the local slaughterhouse. The ovaries were delivered to the laboratory within 2-3 h in a thermos which provided a constant temperature of 25-30oC with physiological saline (0.9%) containing antibiotics. All follicles in the 3-8 mm range on the ovaries were aspirated using 20 G needle. The collected follicle fluid was filtered through filters with a pore diameter of 70 micrometers. Cells remaining in the filter were washed with OPU medium and transferred to the petri dishes. Fluids were examined under a stereomicroscope. The cumulus-oocyte complexes were classified, and A and B quality oocytes were included to the study (A, B, C, and D quality COC). Oocytes aspirated from the ovaries and collected later on were incubated in IVM medium for 22 h. After maturation, it was taken into IVF medium, semen was added and incubated for 20-22 h. Possible zygotes to be taken to the culture stage were transferred to culture (IVC) drops with (L-ergothioneine 100 µL/mL (n:121) added and without antioxidant (control (n:124)), and kept in the incubator for 6-7 days. Evaluated on the 7th day. differences in in vitro embryo production stages were evaluated with the Chi-square test. The study was run in 5 replicates each time, with at least 20 possible zygotes for per group being cultured. It was determined that 262 (87.33%) of a total of 300 oocytes undergoing in vitro maturation were matured. It was determined that 245 of the mature oocytes were fertilized (93.51%). The cleavage rates of the groups were determined as 87.60% and 86.29%, respectively. Eighty-two (33.47%) blastocysts were obtained from 245 zygotes taken into the culture stage, and the blastocyst rates in the groups were found to be 40.50% and 26.61%, respectively. After the study, it was determined that the statistical difference between L-ergothioneine and control in cleavage rates was insignificant (P > 0.05) and blastocyst rates was significant (P < 0.05) Discussion: Oxygen content above normal ratios can increase the formation of reactive oxygen species (ROS), particularly hydrogen peroxide (H2O2), hydroxyl radical (HO·), and peroxyl radicals (ROO·). The increased rate of ROS negatively affects the success of IVP in mammalian embryos. It was observed that L-ergothioneine, which has high antioxidant activity, improved blastocyst development rates, and higher blastocyst rates could be achieved compared to the control group. By investigating the use of L-ergothioneine in different doses, it was thought that the dose with the highest antioxidant activity could be added to the culture medium in in vitro embryo production and more blastocysts could be produced.
Yazarlar (2)
1
Sakine Ulkum Cizmeci
2
Mehmet Burakalp Yusuflu
Anahtar Kelimeler
antioxidant
blastocyst
in vitro
L-ergothioneine
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Acta Scientiae Veterinariae
Q4
SJR Skoru0,155
H-Index20
YayıncıUniversidade Federal do Rio Grande do Sul
ÜlkeBrazil
Veterinary (miscellaneous) (Q4)
Metrikler
2
Atıf
2
Yazar
4
Anahtar Kelime