Scopus
YÖKSİS DOI Eşleşti
SJR Q1
Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos
Theriogenology · Ocak 2025
YÖKSİS Kayıtları
Putrescine Supplementation Improves the Developmental Competence of In Vitro Produced Bovine Embryos
Theriogenology · 2025 SCI-Expanded
Doç. Dr. HASAN ALKAN →
Putrescine Supplementation Improves the Developmental Competence of In Vitro Produced Bovine Embryos
Theriogenology · 2025 SCI-Expanded
Dr. Öğr. Üyesi GONCA SÖNMEZ →
Putrescine Supplementation Improves the Developmental Competence of In Vitro Produced Bovine Embryos
Theriogenology · 2025 SCI-Expanded
Arş. Gör. MUHAMMED HÜDAİ ÇULHA →
Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos
Theriogenology · 2025 SCI-Expanded
Doç. Dr. FATMA SATILMIŞ →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Protective effect of esterified glucomannan on aflatoxin inducedchanges in testicular function sperm quality and seminal plasmabiochemistry in rams
2014 ISSN: 0093-691X SSCI
Prof. Dr. EMRAH SUR →
Protective effect of esterified glucomannan on aflatoxin induced changes in testicular function sperm quality and seminal plasma biochemistry in rams
2014 ISSN: 0093691X SCI-Expanded
Prof. Dr. NURCAN DÖNMEZ →
The influence of trehalose taurine cysteamine and hyaluronan on ram semen
2007 ISSN: 0093691X SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Effect of glutamine and sugars after bull spermatozoa cryopreservation
2011 ISSN: 0093691X SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Detection of Brucella canis from inguinal lymph nodes of naturally infected dogs by PCR
2010 ISSN: 0093691X SSCI
Prof. Dr. UÇKUN SAİT UÇAN →
Protective effect of esterified glucomannan on aflatoxin induced changes in testicular function sperm quality and seminal plasma biochemistry in rams
2014 ISSN: 0093691X SSCI
Prof. Dr. HASAN HÜSEYİN DÖNMEZ →
DNA Damaging Effect of Paclitaxel in the Epididymal Sperms as a Chemotherapeutic Agent and Possible Remedies to Prevent this Effect: A Study on Reproductive Potential of Male Cancer Patients of Reproductive Age
2019 ISSN: 0093-691X SCI Q1
Prof. Dr. MUSTAFA NUMAN BUCAK →
Protective effect of esterified glucomannan on aflatoxin-induced changes in testicular function, sperm quality, and seminal plasma biochemistry in rams
2014 ISSN: 0093-691X SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
The effect of anti-müllerian hormone and progesterone concentrations on superovulation response and embryo yield in goats
2020 ISSN: 0093-691X SCI
Doç. Dr. KÜBRA KARAKAŞ ALKAN →
Chronological echocardiographic evaluation of left ventricular systolic and diastolic function in term and premature neonatal calves
2020 ISSN: 0093-691X SCI-Expanded Q1
Doç. Dr. MERVE İDER →
Chronological echocardiographic evaluation of left ventricular systolic and diastolic function in term and premature neonatal calves
2020 ISSN: 0093-691X SCI-Expanded
Doç. Dr. AMİR NASERİ →
The pharmacokinetics of letrozole and its effect on gonadotropins in anestrous ewes
2021 ISSN: 0093-691X SCI-Expanded Q1
Doç. Dr. HASAN ALKAN →
The pharmacokinetics of letrozole and its effect on gonadotropins in anestrous ewes
2021 ISSN: 0093-691X SCI-Expanded Q1
Prof. Dr. KAMİL ÜNEY →
Serum sRAGE and sE-selectin levels are useful biomarkers of lung injury and prediction of mortality in calves with perinatal asphyxia
2022 ISSN: 0093-691X SCI-Expanded Q1
Doç. Dr. MERVE İDER →
The pharmacokinetics of letrozole and its effect on gonadotropins in anestrous ewes
2021 ISSN: 0093-691X SCI-Expanded
Prof. Dr. İBRAHİM AYDIN →
Serum sRAGE and sE-selectin levels are useful biomarkers of lung injury and prediction of mortality in calves with perinatal asphyxia
2022 ISSN: 0093-691X SCI Q1
Prof. Dr. KAMİL ÜNEY →
Serum sRAGE and sE-selectin levels are useful biomarkers of lung injury and prediction of mortality in calves with perinatal asphyxia
2022 ISSN: 0093-691X SCI-Expanded
Prof. Dr. MAHMUT OK →
The effects of glutathione supplementation on post-thawed Turkey semen quality and oxidative stress parameters and fertilization, and hatching potential
2022 ISSN: 0093-691X SCI-Expanded
Prof. Dr. MUSTAFA NUMAN BUCAK →
Effect of strategies to increase progesterone levels on fertility of bovine embryo transfer recipients - A meta-analysis
2024 ISSN: 0093-691X SCI-Expanded Q1
Doç. Dr. HASAN ALKAN →
Effect of strategies to increase progesterone levels on fertility of bovine embryo transfer recipients - A meta-analysis
2024 ISSN: 0093-691X SCI-Expanded Q1
Doç. Dr. FATMA SATILMIŞ →
Makale Bilgileri
Dergi
Theriogenology
ISSN0093691X
Yayın TarihiOcak 2025
Cilt / Sayfa231 · 133-143
Scopus ID2-s2.0-85207146982
Özet
The aim of this study was to investigate the effect of putrescine, anti-apoptotic, antioxidant, and a cell proliferation stimulant, on embryo development and quality by supplementing it to in vitro culture medium. In this study, oocytes were obtained from the ovaries of Holstein cattle. Following maturation and fertilization, the presumptive zygotes were randomly assigned to two groups. The first group (Putrescine, n = 435) was supplemented with putrescine at a concentration of 0.5 mM to in vitro culture. The second group (n = 407) was maintained under standard culture conditions without any supplementations to the medium. Following the determination of the developmental stages of the embryos, only those in the blastocyst stage were subjected to differential staining and the cell numbers of the embryos were determined. Moreover, the TUNEL assay was employed to ascertain the extent of cell death and the apoptotic index in the embryos. Additionally, the levels of ROS were determined in the embryos. Furthermore, gene expression analyses were conducted on blastocyst-stage embryos to ascertain the potential of putrescine supplementation in embryo development along specific pathways. Following in vitro culture, the blastocyst formation rate was 44.37 % in the putrescine group and 32.97 % in the control group (P < 0.05). The counts of ICM (60.60 ± 15.79 vs 50.73 ± 16.74), TE (117.70 ± 23.67 vs 94.0 ± 22.46), and TCC (178.30 ± 26.15 vs 144.73 ± 26.86) were found to be statistically higher in blastocysts developing after putrescine supplementation compared to the control group. Furthermore, the number of apoptotic cells (7.69 ± 2.17 vs 9.96 ± 3.99) and the apoptotic index (5.07 % vs 8.01 %) were found to be lower in the putrescine group in comparison to the control group. Nevertheless, it was established that the ROS level in the control group was approximately two-fold higher than in the putrescine group (P < 0.05). The findings also revealed that putrescine up-regulated the gene expression of SOD, GPX4, CAT, BCL2, NANOG and GATA3 while simultaneously down-regulating the BAX expression level. In conclusion, the supplementation of putrescine to the culture medium during in vitro bovine embryo production was found to contribute to the improvement of embryo quality and early embryonic development.
Yazarlar (8)
1
Kubra Karakas Alkan
2
Fatma Satilmiş
3
Gonca Sönmez
4
Yunus Emre Deniz
5
M. Hüdai Çulha
6
Muhammed Furkan Ciftci
ORCID: 0000-0001-8333-6500
7
Omer Faruk Yesilkaya
ORCID: 0000-0002-7721-2576
8
Hasan Alkan
ORCID: 0000-0001-8332-5334
Anahtar Kelimeler
Apoptosis
Embryo development
Gene expression
Putrescine
ROS
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Theriogenology
Q1
SJR Skoru0,780
H-Index160
YayıncıElsevier Inc.
ÜlkeUnited States
Animal Science and Zoology (Q1)
Equine (Q1)
Food Animals (Q1)
Small Animals (Q1)
Metrikler
5
Atıf
8
Yazar
5
Anahtar Kelime