Scopus
YÖKSİS Eşleşti
Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos
Theriogenology · Ocak 2025
YÖKSİS Kayıtları
Putrescine Supplementation Improves the Developmental Competence of In Vitro Produced Bovine Embryos
Theriogenology · 2025 SCI-Expanded
DOÇENT HASAN ALKAN →
Putrescine Supplementation Improves the Developmental Competence of In Vitro Produced Bovine Embryos
Theriogenology · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ GONCA SÖNMEZ →
Putrescine Supplementation Improves the Developmental Competence of In Vitro Produced Bovine Embryos
Theriogenology · 2025 SCI-Expanded
ARAŞTIRMA GÖREVLİSİ MUHAMMED HÜDAİ ÇULHA →
Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos
Theriogenology · 2025 SCI-Expanded
DOÇENT FATMA SATILMIŞ →
Makale Bilgileri
DergiTheriogenology
Yayın TarihiOcak 2025
Cilt / Sayfa231 · 133-143
Scopus ID2-s2.0-85207146982
Özet
The aim of this study was to investigate the effect of putrescine, anti-apoptotic, antioxidant, and a cell proliferation stimulant, on embryo development and quality by supplementing it to in vitro culture medium. In this study, oocytes were obtained from the ovaries of Holstein cattle. Following maturation and fertilization, the presumptive zygotes were randomly assigned to two groups. The first group (Putrescine, n = 435) was supplemented with putrescine at a concentration of 0.5 mM to in vitro culture. The second group (n = 407) was maintained under standard culture conditions without any supplementations to the medium. Following the determination of the developmental stages of the embryos, only those in the blastocyst stage were subjected to differential staining and the cell numbers of the embryos were determined. Moreover, the TUNEL assay was employed to ascertain the extent of cell death and the apoptotic index in the embryos. Additionally, the levels of ROS were determined in the embryos. Furthermore, gene expression analyses were conducted on blastocyst-stage embryos to ascertain the potential of putrescine supplementation in embryo development along specific pathways. Following in vitro culture, the blastocyst formation rate was 44.37 % in the putrescine group and 32.97 % in the control group (P < 0.05). The counts of ICM (60.60 ± 15.79 vs 50.73 ± 16.74), TE (117.70 ± 23.67 vs 94.0 ± 22.46), and TCC (178.30 ± 26.15 vs 144.73 ± 26.86) were found to be statistically higher in blastocysts developing after putrescine supplementation compared to the control group. Furthermore, the number of apoptotic cells (7.69 ± 2.17 vs 9.96 ± 3.99) and the apoptotic index (5.07 % vs 8.01 %) were found to be lower in the putrescine group in comparison to the control group. Nevertheless, it was established that the ROS level in the control group was approximately two-fold higher than in the putrescine group (P < 0.05). The findings also revealed that putrescine up-regulated the gene expression of SOD, GPX4, CAT, BCL2, NANOG and GATA3 while simultaneously down-regulating the BAX expression level. In conclusion, the supplementation of putrescine to the culture medium during in vitro bovine embryo production was found to contribute to the improvement of embryo quality and early embryonic development.
Yazarlar (8)
1
Kubra Karakas Alkan
2
Fatma Satilmiş
3
Gonca Sönmez
4
Yunus Emre Deniz
5
M. Hüdai Çulha
6
Muhammed Furkan Ciftci
ORCID: 0000-0001-8333-6500
7
Omer Faruk Yesilkaya
ORCID: 0000-0002-7721-2576
8
Hasan Alkan
ORCID: 0000-0001-8332-5334
Anahtar Kelimeler
Apoptosis
Embryo development
Gene expression
Putrescine
ROS
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey