Scopus
YÖKSİS DOI Eşleşti
SJR Q1
Preparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome c
Materials Science and Engineering C · Ocak 2019
YÖKSİS Kayıtları
Preparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome c
Materials Science and Engineering: C · 2019 SCI-Expanded
Prof. Dr. AHMED NURİ KURŞUNLU →
Preparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome c
Materials Science and Engineering: C · 2019 SCI-Expanded
Prof. Dr. MUSTAFA ÖZMEN →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Novel Humic Acid Bonded Magnetite Nanoparticles for Protein Immobilization
2014 ISSN: 0928-4931 SCI-Expanded 5 atıf
Prof. Dr. MUSTAFA ÖZMEN →
Novel humic acid bonded magnetite nanoparticles for protein immobilization
2014 ISSN: 09284931 SCI-Expanded
Prof. Dr. ESRA MALTAŞ ÇAĞIL →
Novel humic acid bonded magnetite nanoparticles for protein immobilization
2014 ISSN: 09284931 SCI-Expanded
Prof. Dr. SALİH ZEKİ BAŞ →
Two novel calixarene functionalized iron oxide magnetite nanoparticles as a platform for magnetic separation in the liquid liquid solid liquid extraction of oxyanions
2013 ISSN: 0928-4931 SCI-Expanded 10 atıf
Öğr. Gör. FATİH ÖZCAN →
Preparation and application of calix 4 arene grafted magnetite nanoparticles for removal of dichromate anions
2009 ISSN: 09284931 SCI-Expanded 26 atıf
Öğr. Gör. FATİH ÖZCAN →
Spectrofluorometric and thermal gravimetric study on binding interaction of thiabendazole with hemoglobin on epoxy functionalized magnetic nanoparticles
2015 ISSN: 09284931 SCI-Expanded
Prof. Dr. ESRA MALTAŞ ÇAĞIL →
Fluorescent labelling of DNA on superparamagnetic nanoparticles by a perylene bisimide derivative for cell imaging
2015 ISSN: 09284931 SCI-Expanded
Prof. Dr. ESRA MALTAŞ ÇAĞIL →
Fluorescent labelling of DNA on superparamagnetic nanoparticles by a perylene bisimide derivative for cell imaging
2015 ISSN: 09284931 SCI-Expanded
Prof. Dr. MUSTAFA ÖZMEN →
Spectrofluorometric and thermal gravimetric study on binding interaction of thiabendazole with hemoglobin on epoxy functionalized magnetic nanoparticles
2015 ISSN: 09284931 SCI-Expanded
Prof. Dr. MUSTAFA ÖZMEN →
Fluorescent labelling of DNA on superparamagnetic nanoparticles by a perylene bisimide derivative for cell imaging
2015 ISSN: 09284931 SCI-Expanded
Dr. Öğr. Üyesi PEMBEGÜL UYAR ARPACI →
Two novel calixarene functionalized iron oxide magnetite nanoparticles as a platform for magnetic separation in the liquid liquid solid liquid extraction of oxyanions
2013 ISSN: 09284931 SSCI 4 atıf
Prof. Dr. MUSTAFA YILMAZ →
Preparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome c
2019 ISSN: 0928-4931 SCI-Expanded
Prof. Dr. AHMED NURİ KURŞUNLU →
A new pollen-derived microcarrier for pantoprazole delivery
2017 ISSN: 09284931 SCI-Expanded
Prof. Dr. İDRİS SARGIN →
In situ chitin isolation from body parts of a centipede and lysozyme adsorption studies
2017 ISSN: 09284931 SCI-Expanded
Prof. Dr. İDRİS SARGIN →
Newly isolated sporopollenin microcages from Platanus orientalis pollens as a vehicle for controlled drug delivery
2017 ISSN: 09284931 SCI-Expanded
Prof. Dr. İDRİS SARGIN →
Three-dimensional chitin rings from body segments of a pet diplopod species: Characterization and protein interaction studies
2016 ISSN: 09284931 SCI-Expanded
Prof. Dr. İDRİS SARGIN →
Production of a new platform based calixarene nanofiber for controlled release of the drugs
2019 ISSN: 0928-4931 SCI-Expanded
Prof. Dr. ESRA MALTAŞ ÇAĞIL →
Production of a new platform based calixarene nanofiber for controlled release of the drugs
2019 ISSN: 0928-4931 SCI-Expanded
Prof. Dr. ESRA MALTAŞ ÇAĞIL →
Makale Bilgileri
ISSN09284931
Yayın TarihiOcak 2019
Cilt / Sayfa94 · 886-893
Scopus ID2-s2.0-85054826709
Özet
This work presents the immobilization of trypsin on the synthesized pillar[5]arene derivative (P5) containing ten iodo-terminals. The characterization has been carried out by Thermogravimetric Analysis (TGA), Fourier Transform Infrared (FTIR) and Raman spectra, Scanning Electron Microscopy (SEM) and Zeta Potential. Furthermore, Cytochrome c (Cyt c) was chosen as a model protein for evaluation of the performance of the pillar[5]arene-immobilized trypsin (P5-T), and its microwave-assisted digestion conditions were investigated by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for 15 second. The peptide numbers of 10 and sequence coverage of 93% for 15 second microwave-assisted digestion were obtained for P5-T. The results can be further applied in future proteomics studies due to high efficiency, good reproducibility and stability of the pillar[5]arene-immobilized trypsin.
Yazarlar (3)
1
Keziban Atacan
2
Ahmed Nuri Kursunlu
3
Mustafa Ozmen
ORCID: 0000-0001-5117-9168
Anahtar Kelimeler
Cytochrome c
Immobilization
Microwave digestion
Pillar[5]arene
Trypsin
Kurumlar
Sakarya Üniversitesi
Serdivan Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Materials Science and Engineering C
Q1
SJR Skoru1,086
YayıncıElsevier B.V.
ÜlkeNetherlands
Bioengineering (Q1)
Biomaterials (Q1)
Condensed Matter Physics (Q1)
Materials Science (miscellaneous) (Q1)
Mechanical Engineering (Q1)
Mechanics of Materials (Q1)
Metrikler
37
Atıf
3
Yazar
5
Anahtar Kelime