Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q3
Determination of vibrio parahaemolyticus in seafoods using direct plate counting, quantitative loop-mediated isothermal amplification and propidium monoazide-qLAMP
Ankara Universitesi Veteriner Fakultesi Dergisi · Ocak 2020
YÖKSİS Kayıtları
Determination of Vibrio parahaemolyticus in seafoods using direct plate counting, quantitative loop-mediated isothermal amplification and propidium monoazide-qLAMP
Ankara Universitesi Veteriner Fakultesi Dergisi · 2020 SCI-Expanded
Prof. Dr. YUSUF DOĞRUER →
Determination of Vibrio parahaemolyticus in seafoods using direct plate counting, quantitative loop-mediated isothermal amplification and propidium monoazide-qLAMP
Ankara Universitesi Veteriner Fakultesi Dergisi · 2020 SCI-Expanded
Doç. Dr. ARİFE EZGİ TELLİ →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Serum ceruloplasmin and paraoxonase-1 levels in ovariectomized and ovariohysterectomized dogs
2015 ISSN: 1300-0861 SCI-Expanded
Doç. Dr. KÜBRA KARAKAŞ ALKAN →
The role of different trehalose concentrations and cooling rates in freezing of ram semen
2009 ISSN: 1300-0861 SCI-Expanded 5 atıf
Prof. Dr. MUSTAFA NUMAN BUCAK →
Effects of dietary sepiolite on performance egg quality and some blood parameters in laying hens
2016 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. SUZAN YALÇIN →
Effects of brooder machine rearing method in the first week on fattening performance, carcass characteristics, meat quality and some blood parameters of Pekin ducks
2015 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. SUZAN YALÇIN →
The Determination of Situation and Breed Charecteristics of Turkish Rahvan Horse in Turkey
2010 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. AYTEKİN GÜNLÜ →
Financial losses due to lameness in dairy herds in Burdur, Kirklareli and Konya Provinces Turkey
2010 ISSN: 1300-0861 SCI
Prof. Dr. AYTEKİN GÜNLÜ →
A Comparison of Egg Quality Characteristics of Partridge and Pheasant Eggs P colchicus A graeca
2010 ISSN: 1300-0861 SSCI
Prof. Dr. AYTEKİN GÜNLÜ →
Effects of dietary yeast cell wall on performance egg quality and humoral immune response in laying hens
2014 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. SUZAN YALÇIN →
Effects of hypothyroidism and hyperthyroidism on hematological parameters in rats
2016 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. ERCAN KESKİN →
Kuru döneme çıkarken enfekte ve sağlıklı meme loblarında California Mastitis Test ve somatik hücre sayısı ile bakteriyolojik muayene sonuçlarının karşılaştırılması
2014 ISSN: 1300-0861 SCI-Expanded
Doç. Dr. HASAN ALKAN →
Serum ceruloplasmin and paraoxonase-1 levels in ovariectomized and ovariohysterectomized dogs
2015 ISSN: 1300-0861 SCI-Expanded
Doç. Dr. HASAN ALKAN →
Investigation of mycotoxin residues in poultry feeds by LC MS/MS method
2017 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. HALİS UĞUZ →
Chewing lice (Phthiraptera: Amblycera, Ischnocera) of some aquatic birds in Konya province, Turkey, new records for Turkish fauna
2017 ISSN: 1300-0861 SCI-Expanded
Doç. Dr. ONUR CEYLAN →
The effects of various essential oils Carvacrol Cinnamaldehyde Thymol having antimicrobial properties that are applied to broilerlitter on litter hygiene and broiler performance
2011 ISSN: 1300-0861 SCI
Prof. Dr. İSKENDER YILDIRIM →
LimuzinXJersey melezi ve Holştayn ırkı tosunlarda optimal besi süresinin tespiti üzerine bir araştırma
1996 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. AYTEKİN GÜNLÜ →
Histomorphometric examination of the pineal gland in foals and adult horses
2018 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. SADULLAH BAHAR →
The light microscopic investigation of the effects of in-ovo administered bisphenol A (BPA) on the development of testes
2018 ISSN: 1300-0861 SCI-Expanded
Prof. Dr. EMRAH SUR →
Histomorphometric examination of the pineal gland in foals and adult horses.
2018 ISSN: 1300-0861 SCI-Expanded Q4
Prof. Dr. SADULLAH BAHAR →
Makale Bilgileri
ISSN13000861
Yayın TarihiOcak 2020
Cilt / Sayfa67 · 349-355
Scopus ID2-s2.0-85090515404
Erişim🔓 Açık Erişim
Özet
One of the most challenging aspects in culture independent methods for foodborne pathogens’ detection is discrimination of dead and live microorganisms. This study aimed to determine the Vibrio parahaemolyticus in seafoods via direct plate counting (DPC) and toxR-based quantitative loop-mediated isothermal amplification (qLAMP) and to discriminate dead and live cells using propidium monoazide (PMA)-qLAMP. A total of 200 samples including finfishes (n= 100) and shrimps (n= 100), representing the Mediterranean, Black and Aegean sea were collected from supermarkets and fish markets of Konya-Turkey. qLAMP was performed in a Real-Time Turbidimeter and the time threshold (tt) values were yielded in 60 minutes. On DPC, the colonies grown on TCBS Agar was further confirmed by conventional PCR based from gyrB1 gene of Vibrio spp. and toxR gene of V. parahaemolyticus. Virulence property of the isolates were determined by tdh based qLAMP. The detection limit of the qLAMP was 1.2×104 CFU/g in artificially contaminated seafoods. DPC, qLAMP and PMA-qLAMP detected V. parahaemolyticus in 8 (4%), 12 (6%) and 12 (6%) samples, respectively. The CFUs of V. parahaemolyticus detected in qLAMP (5.96±0.10 log10 CFU/ml) and PMA-qLAMP (4.71±0.13 log10 CFU/ml) were higher than those of DPC (1.99±0.44 log10 CFU/ml) (P<0.05). The mean tt reduction in PMA treated samples was 1.25±0.38 log10 CFU/sample. The tdh gene was not detected in any of the isolates. In conclusion, the toxR-based PMA-qLAMP method could be an alternative to be used more widely and effective assay for the quantification of live V. parahaemolyticus in seafoods.
Yazarlar (2)
1
Y. Doğruer
2
A. Ezgi Telli
Anahtar Kelimeler
DPC
Propidium monoazide
QLAMP
VBNC
V. parahaemolyticus
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Ankara Universitesi Veteriner Fakultesi Dergisi
Q3
OA
SJR Skoru0,271
H-Index19
YayıncıAnkara University
ÜlkeTurkey
Animal Science and Zoology (Q3)
Veterinary (miscellaneous) (Q3)