Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Optimization of callus cultures at Echinacea purpurea L. for the amount of caffeic acid derivatives
Electronic Journal of Biotechnology · Mayıs 2021
YÖKSİS Kayıtları
Optimization of callus cultures at Echinacea purpurea L. for the amount of caffeic acid derivatives
Electronic Journal of Biotechnology · 2021 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MÜNÜRE TANUR ERKOYUNCU →
Optimization of callus cultures at Echinacea purpurea L. for the amount of caffeic acid derivatives
ELECTRONIC JOURNAL OF BIOTECHNOLOGY · 2021 SCI-Expanded
PROFESÖR MUSTAFA YORGANCILAR →
Makale Bilgileri
DergiElectronic Journal of Biotechnology
Yayın TarihiMayıs 2021
Cilt / Sayfa51 · 17-27
Scopus ID2-s2.0-85103309128
Erişim🔓 Açık Erişim
Özet
Background: In order to produce an effective callus in Echinacea purpurea L.; determination of the explant type and growth regulators that best respond to callus induction and the optimization of the culture conditions to increase the amount of caffeic acid derivatives (CADs) in the obtained callus. CADs contents of callus cultures of E. purpurea were evaluated by establishing an effective callus induction system in vitro. Results: Various medium containing different growth regulators were tested using leaf, petiole, cotyledon and root as the explants. The best callus development was achieved in MS medium with 1.0 mg l−1 2,4-D + 2.0 mg l−1 BAP in leaf, 1.0 mg l−1 NAA + 0.5 mg l−1 TDZ in petiole, 2.0 mg l−1 NAA + 1.0 mg l−1 TDZ in cotyledon and 0.5 mg l−1 NAA + 0.5 mg l−1 BAP in roots. Upon optimisation of callus growth, each type of explant was cultured for 4, 6, 8 and 10 weeks in medium for the analyses of caftaric acid, chlorogenic acid, caffeic acid and chicoric acid contents. The highest amounts of caftaric acid (4.11 mg/g) and chicoric acid (57.89 mg/g) were found from petiole explants and chlorogenic acid (8.83 mg/g) from root explants at the end of the 10-week culture time. Conclusions: As a result of the present study, the production of caffeic acid derivatives was performed by providing the optimization of E. purpurea L. callus cultures. Effective and repeatable protocols established in this study may offer help for further studies investigating the production of caffeic acid derivatives in vitro. How to cite: Tanur Erkoyuncu M, Yorgancilar M. Optimization of callus cultures at Echinacea purpurea L. for the amount of caffeic acid derivatives. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.02.003.
Yazarlar (2)
1
Münüre Tanur Erkoyuncu
ORCID: 0000-0001-5004-4771
2
Mustafa Yorgancilar
ORCID: 0000-0003-4938-8547
Anahtar Kelimeler
Caffeic acid derivatives
Callus culture
Callus induction
Cotyledon explant
Culture time
Echinacea
Explant type
Growth regulators
Leaf explant
Petiole explant
Root explant
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
10
Atıf
2
Yazar
11
Anahtar Kelime