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Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus

Journal of Fluorescence · Eylül 2021

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YÖKSİS Kayıtları
Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus
Journal of Fluorescence · 2021 SCI-Expanded
PROFESÖR İDRİS SARGIN →
Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus
Journal of Fluorescence · 2021 SCI-Expanded
PROFESÖR GÜLŞİN ARSLAN →
Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus
Journal of Fluorescence · 2021 SCI-Expanded
PROFESÖR SERDAR KARAKURT →
Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus
Journal of Fluorescence · 2021 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ SİNAN ALKAN →

Makale Bilgileri

DergiJournal of Fluorescence
Yayın TarihiEylül 2021
Cilt / Sayfa31 · 1461-1473
Özet In the study, fluorescent imaging of live cells was performed using fluorescent carbon quantum dots derived from edible mushrooms species; Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus as a fluorophore agent. Carbon quantum dots were synthesized through a facile and low-cost method based on microwave irradiation of dried mushroom samples in hydrogen peroxide solution under optimized conditions (microwave energy, solution type, duration of microwave treatment, amount of mushroom). Upon purification with centrifugation, microfiltration, and dialysis, the lyophilized carbon quantum dots were identified through UV–visible, fluorescence and FT-IR, X-ray photoelectron spectroscopy, X-ray diffraction, high-resolution transmission electron microscopy, and quantum yield calculation. Cell viability assessment of the carbon quantum dots was evaluated against human epithelial cell line PNT1A using the Alamar Blue Assay. In vitro fluorescence cell imaging studies demonstrated that the carbon dots could dynamically penetrate the cell membrane and nuclear membrane and localize in both the cytoplasm and the nucleus.

Yazarlar (4)

1
Idris Sargin
ORCID: 0000-0003-3785-9575
2
Serdar Karakurt
3
Sinan Alkan
4
Gulsin Arslan

Anahtar Kelimeler

Carbon quantum dot Cellular imaging Edible mushroom PNT1A cell line

Kurumlar

Selçuk Üniversitesi
Selçuklu Turkey

Metrikler

10
Atıf
4
Yazar
4
Anahtar Kelime

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