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A new BODIPY/nanoparticle/Ni affinity system for binding of cytochrome c

Applied Surface Science · Eylül 2015

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YÖKSİS Kayıtları
A new BODIPY nanoparticle Ni affinity system for binding of cytochrome c
APPLIED SURFACE SCIENCE · 2015 SCI-Expanded
PROFESÖR MUSTAFA ÖZMEN →
A new BODIPY nanoparticle Ni affinity system for binding of cytochrome c
Applied Surface Science · 2015 SCI-Expanded
PROFESÖR MUSTAFA ÖZMEN →
A new BODIPY nanoparticle Ni affinity system for binding of cytochrome c
Applied Surface Science · 2015 SCI-Expanded
PROFESÖR ESRA MALTAŞ ÇAĞIL →
A new BODIPY nanoparticle Ni affinity system for binding of cytochrome c
Applied Surface Science · 2015 SCI-Expanded
PROFESÖR AHMED NURİ KURŞUNLU →
A new BODIPY nanoparticle Ni affinity system for binding of cytochrome c
Applied Surface Science · 2015 SCI-Expanded
PROFESÖR GÜLŞİN ARSLAN →

Makale Bilgileri

DergiApplied Surface Science
Yayın TarihiEylül 2015
Cilt / Sayfa349 · 811-816
DOI10.1016/j.apsusc.2015.05.082
Scopus ID2-s2.0-84937707022
Özet In this study, 3,5-{Bis[4,4-difluoro, 8-(2,6-diethyl, 1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene)]}benzoylchloride (BODIPY) was synthesized for the improving of a new immobilized metal affinity supporting material. Firstly, the synthesized BODIPY was immobilized on iron oxide superparamagnetic nanoparticles (SPIONs) and then, Ni(II) ions were chelated with the active terminals of BODIPY on nanoparticles surfaces to prepare an immobilized metal affinity (IMA) adsorbent for protein adsorption. The amount of BODIPY coated on SPIONs was about 29.7 μM at 10 mg nanoparticles. 738 μmol of Ni(II) ions were loaded to 10 mg of the SPIONs/BODIPY. The binding amount of cytochrome c was found to be 170 μg to the SPIONs/BODIPY/Ni at pH 7.4. The binding amount of the molecules on SPIONs was analyzed by using UV-vis, fluorescence and atomic absorption spectroscopy. The characterization of the prepared surfaces was performed by FT-IR, SEM and TEM.

Yazarlar (4)

1
Esra Maltas Cagil
2
Ahmed Nuri Kursunlu
3
Gulsin Arslan
4
Mustafa Ozmen
ORCID: 0000-0001-5117-9168

Anahtar Kelimeler

Affinity BODIPY Nanoparticles Protein Separation

Kurumlar

Selçuk Üniversitesi
Selçuklu Turkey

Metrikler

11
Atıf
4
Yazar
5
Anahtar Kelime

Sistemimizdeki Yazarlar

GA
GÜLŞİN ARSLAN
PROFESÖR

Hızlı Erişim

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