Scopus
YÖKSİS Eşleşti
Evaluation of random amplified polymorphic DNA and multiple-locus variable number tandem repeat analyses for Mycoplasma cynos
Journal of Microbiological Methods · Haziran 2019
YÖKSİS Kayıtları
Evaluation of random amplified polymorphic DNA and multiple-locus variable number tandem repeat analyses for Mycoplasma cynos
JOURNAL OF MICROBIOLOGICAL METHODS · 2019 SCI
DOÇENT ASLI BALEVİ →
Evaluation of random amplified polymorphic DNA and multiple-locus variable number tandem repeat analyses for Mycoplasma cynos
JOURNAL OF MICROBIOLOGICAL METHODS · 2019 SCI
PROFESÖR ZAFER SAYIN →
Makale Bilgileri
DergiJournal of Microbiological Methods
Yayın TarihiHaziran 2019
Cilt / Sayfa161 · 1-7
Scopus ID2-s2.0-85064321545
Özet
Mycoplasma spp. can cause diseases of the respiratory system as well as urogenital infections, infertility, and anemia. The members of this genus have a low G + C content compared to other bacteria. Because primers used in the random amplified polymorphic DNA (RAPD) technique are only 10 bp long and have high GC content, this method can be inadequate for genotyping Mycoplasma spp. isolates. The aim of this study was to develop and evaluate multiple-locus variable number tandem repeat analysis (MLVA) and two-primer RAPD (TP-RAPD) procedures for subtyping Mycoplasma cynos isolates. A total of 55 M. cynos isolates obtained from 162 bronchoalveolar lavage fluid samples from shelter and pet dogs were used in this study. Seventy-four tandem repeat regions were detected in the M. cynos genome, and two of these loci were determined to be suitable and used for development of the MLVA scheme. The results of variable number tandem repeat (VNTR) analysis and TP-RAPD-PCR were compared with RAPD-PCR. The discriminatory power of TP-RAPD-PCR (Hunter-Gaston diversity index [HGDI] = 0.84) was higher than those of RAPD-PCR (HGDI = 0.727), VNTR1 (HGDI = 0.8), and VNTR3 (HGDI = 0.757). We observed that the TP-RAPD-PCR and MLVA methods provide clearer data and are more successful in determining genetic diversity, in contrast to the RAPD-PCR method for this species.
Yazarlar (6)
1
Aslı Sakmanoğlu
2
Zafer Sayın
3
Yasemin Pinarkara
4
Ali Uslu
5
Uçkun Sait Uçan
6
O. Erganiş
Anahtar Kelimeler
Bronchoalveolar lavage fluid
MLVA
Mycoplasma cynos
RAPD
TP-RAPD
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
2
Atıf
6
Yazar
5
Anahtar Kelime