Scopus
YÖKSİS Eşleşti
Determination of thermotolerant Campylobacter spp. by viability-qPCR in a poultry slaughterhouse and in retail
European Poultry Science · Ocak 2021
YÖKSİS Kayıtları
Determination of thermotolerant Campylobacter spp. by viability-qPCR in a poultry slaughterhouse and in retail
European Poultry Science · 2021 SCI-Expanded
DOÇENT ARİFE EZGİ TELLİ →
Determination of thermotolerant Campylobacter spp. by viability-qPCR in a
poultry slaughterhouse and in retail
EUROPEAN POULTRY SCIENCE · 2021 SCI-Expanded
PROFESÖR YUSUF DOĞRUER →
Determination of thermotolerant Campylobacter spp. by viability-qPCR in a poultry slaughterhouse and in retail
European Poultry Science · 2021 SCI-Expanded
PROFESÖR GÜRKAN UÇAR →
Determination of thermotolerant Campylobacter spp. by viability-qPCR in a poultry slaughterhouse and in retail
European Poultry Science · 2021 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ GONCA SÖNMEZ →
Determination of thermotolerant Campylobacter spp. by viability-qPCR in a poultry slaughterhouse and in retail
European Poultry Science · 2021 SCI-Expanded
ARAŞTIRMA GÖREVLİSİ GAMZE TURKAL →
Determination of thermotolerant Campylobacter spp. by viability-qPCR in a poultry slaughterhouse and in retail
European Poultry Science · 2021 SCI-Expanded
ARAŞTIRMA GÖREVLİSİ GAMZE TURKAL →
Makale Bilgileri
DergiEuropean Poultry Science
Yayın TarihiOcak 2021
Cilt / Sayfa85
Scopus ID2-s2.0-85122181848
Özet
This study aimed to evaluate the viability of thermotolerant Campylobacter spp. in chicken carcasses during the slaughter process (n = 150) and at retail level (n = 72). All samples were analysed by quantitative PCR (qPCR), propidium monoazide (PMA)-treated qPCR, and direct plate counting (DPC). The quantitative comparisons were performed for assessing viability and culturability according to the values of the samples that were found to be positive in all methods. Due to the underestimation of viable but unculturable (VBNC) or dead cells, PMA-untreated samples, either at different slaughter stages or at retail level, had a higher positivity and quantity rate (p < 0.05) than the PMA-treated and DPC samples. Across the slaughter line, the above mentioned three methods revealed the highest positivity and quantity level during the evisceration stage. After the water-chilling and storage phases, positivity and quantity rates were not comparable as PMA-qPCR and DPC failed to detect the level of contamination not only in terms of cultivability but also in terms of viability as a result of compromised membrane integrity at low temperatures. In conclusion, recording of Campylobacter spp. during the chicken slaughter process by PMA-treated qPCR would provide more realistic quantitative data for poultry meat contamination.
Yazarlar (6)
1
E. Telli
2
Gürkan Uçar
3
Gonca Sönmez
4
Nihat Telli
5
Gamze Turkal
6
Y. Doğruer
Anahtar Kelimeler
Culturability
Direct plate counting
Propidium monoazide
Retail
Slaughter stages
Viability
Kurumlar
Konya Technical University
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey